人角膜内皮细胞自体再生的研究进展

doi:10.3969/j.issn.1674-8115.2023.06.015

摘要/Abstract

摘要：

人角膜内皮细胞（human corneal endothelial cells，HCECs）对于维持角膜透明性非常重要，但胚胎发育后该细胞停滞于细胞周期G1期，常无法进行增殖与再生。伴随着角膜的发育与年龄的增长，HCECs密度不断下降，且全身性因素及眼部疾病又可进一步加剧HCECs的缺失，造成角膜混浊与水肿，最终进展为视力损害。因此，HCECs的再生一直是角膜内皮研究领域的热点。目前，细胞疗法介导的外源性角膜内皮功能重建与内源性HCECs自体再生均已取得了卓有成效的进展，其中HCECs自体再生则是更加便捷且更符合生理学的治疗方案。基于此，该文从手术治疗、基因治疗以及药物治疗这三方面对HCECs自体再生的策略及与之相关的技术进行汇总与分析。

关键词: 人角膜内皮细胞, 细胞增殖, 自体再生, 手术治疗, 基因治疗, 药物治疗

Abstract:

Human corneal endothelial cells (HCECs) are very important for maintaining corneal transparency, but HCECs remain arrested at the G1 phase after embryonic development and could not proliferate and regenerate in vivo. The density of HCECs decreases spontaneously with corneal development and aging, while systemic factors and corneal diseases can further cause a massive loss to HCECs, lead to corneal opacity and edema and ultimately induce vision impairment. Therefore, the regeneration of HCECs has always been a heated topic in the field of corneal endothelial research. Currently, function restoration of exogenous corneal endothelium mediated by cell therapy and autologous regeneration of endogenous HCECs have made amazing breakthroughs, with endogenous HCECs autologous regeneration being a more convenient and physiological treatment option. This review summarizes and analyzes the strategies and related techniques that are currently applied to the autologous regeneration of HCECs in aspects of operative treatment, gene therapy and pharmacological treatment.

Key words: human corneal endothelial cell (HCEC), cell proliferation, autologous regeneration, operative treatment, gene therapy, pharmacological treatment

中图分类号:

陈瑾, 傅瑶. 人角膜内皮细胞自体再生的研究进展[J]. 上海交通大学学报（医学版）, 2023, 43(6): 775-780.

CHEN Jin, FU Yao. Research progress in autologous regeneration of human corneal endothelial cells[J]. Journal of Shanghai Jiao Tong University (Medical Science), 2023, 43(6): 775-780.

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