七氟醚对小鼠空间学习和记忆能力的影响

doi:10.3969/j.issn.1674-8115.2011.02.014

›› 2011, Vol. 31 ›› Issue (2): 182-.doi: 10.3969/j.issn.1674-8115.2011.02.014

七氟醚对小鼠空间学习和记忆能力的影响

徐敏, 陆志俊, 张富军

上海交通大学医学院附属瑞金医院麻醉科, 上海 200025

出版日期:2011-02-28 发布日期:2011-03-01
通讯作者: 张富军, 电子信箱: fujunzhang1964@yahoo.com.cn。
作者简介:徐敏（1977—）, 女, 主治医师, 学士；电子信箱: xumin-lw@hotmail.com。

Effects of sevoflurane on spatial learning and memory in mice

XU Min, LU Zhi-jun, ZHANG Fu-jun

Department of Anesthesiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China

Online:2011-02-28 Published:2011-03-01

摘要/Abstract

摘要：

目的通过Morris水迷宫实验，评价七氟醚麻醉对小鼠空间学习和记忆能力的影响。方法将24只C57BL/6品系小鼠分为麻醉组（n=12）和对照组（n=12），两组小鼠分别给予七氟醚麻醉120 min和吸纯氧120 min，均于次日开始进行Morris水迷宫实验，包括4 d训练和1 d 探索实验。训练期记录平均游泳速度、潜伏期和游泳距离。探索期记录穿越平台次数、平台象限滞留时间和平台象限滞留时间百分比，并进行统计学分析。结果训练期第2～4天，麻醉组小鼠潜伏期明显短于对照组（P<0.05）；在训练期第2天，麻醉组小鼠的游泳距离也明显短于对照组（P<0.05）。探索实验显示，与对照组比较，麻醉组小鼠平台象限滞留时间和平台象限滞留时间百分比明显增加（P<0.05）。结论七氟醚麻醉具有增强小鼠空间学习和记忆能力的作用。

关键词: 七氟醚, Morris水迷宫, 学习能力, 空间记忆

Abstract:

Objective To observe the effects of sevoflurane anesthesia on spatial learning and memory in mice. Methods Twentyfour C57BL/6 mice were divided into anesthesia group and control group (n=12). Since the following day, Morris water maze was performed on mice in both groups, including 4 trail days and 1 probe day. Latency, distance and average swimming velocity were recorded during trail days. Times across the platform, time during platform quadrant and time percent during platform quadrant were recorded on probe day. The data were statistically analyzed. Results From trail day 2-4, latencies of mice in anesthesia group were significantly shorter than those in control group (P<0.05). On trail day 2, distance of mice in anesthesia group was also significantly shorter than that of control group (P<0.05). On probe day, time during platform quadrant and time percent during platform quadrant in anesthesia group were significantly longer than those in control group (P<0.05). Conclusion Sevoflurane anesthesia improves not only learning ability but also spatial memory in mice.

Key words: sevoflurane, Morris water maze, learning ability, spatial memory

徐敏, 陆志俊, 张富军. 七氟醚对小鼠空间学习和记忆能力的影响[J]. , 2011, 31(2): 182-.

XU Min, LU Zhi-jun, ZHANG Fu-jun. Effects of sevoflurane on spatial learning and memory in mice[J]. , 2011, 31(2): 182-.

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七氟醚对小鼠空间学习和记忆能力的影响

Effects of sevoflurane on spatial learning and memory in mice

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