上海交通大学学报（医学版）

›› 2012, Vol. 32 ›› Issue (4): 467-.doi: 10.3969/j.issn.1674-8115.2012.04.021

• 论著（基础研究） • 上一篇    下一篇

丙戊酸对人卵巢A2780细胞增殖及血管生成和转移相关因子表达的影响

杜华文1, 荣风年2   

  1. 1.上海交通大学 医学院附属第三人民医院妇产科, 上海 201900； 2.山东省千佛山医院妇产科, 济南 250014
  • 出版日期:2012-04-28 发布日期:2012-04-27
  • 通讯作者: 荣风年, 电子信箱: fnyong@163.com。
  • 作者简介:杜华文（1975—）, 女, 主治医师, 硕士；电子信箱: huawen.du@163.com。

Impact of valproic acid on growth and expression of angiogenesis and metastasis-related factors in human ovarian carcinoma A2780 cells

DU Hua-wen1, RONG Feng-nian2   

  1. 1.Department of Obstetrics and Gynecology, the Third People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201900, China；2.Department of Obstetrics and Gynecology, Shandong Province Qianfoshan Hospital, Jinan 250014, China
  • Online:2012-04-28 Published:2012-04-27

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摘要：

目的 研究丙戊酸（VPA）对人卵巢癌A2780细胞生长、血管生成和转移相关因子表达的影响。方法 用不同浓度的VPA处理体外培养的人卵巢癌A2780细胞，采用MTT比色法、流式细胞仪和免疫组织化学检测细胞生长和凋亡、血管内皮生长因子（VEGF）和基质金属蛋白酶-9（MMP-9）蛋白的表达变化。结果 经VPA处理后，A2780细胞的细胞形态发生明显变化，细胞生长能力降低，并呈现浓度和时间依赖性，VEGF和MMP-9蛋白表达均降低。结论 VPA可以抑制卵巢癌A2780细胞生长，并诱导人卵巢癌细胞凋亡，下调VEGF和MMP-9蛋白表达。VEGF和MMP-9蛋白表达下调提示VPA可能有抑制肿瘤血管生成和转移作用。

关键词: 卵巢肿瘤, 丙戊酸, 血管内皮生长因子, 基质金属蛋白酶, 细胞调亡

Abstract:

Objective To investigate the impact of valproic acid (VPA) on growth and expression of angiogenesis and metastasisrelated factors in human ovarian carcinoma A2780 cells. Methods Human ovarian carcinoma A2780 cells cultured in vitro were treated with different concentrations of VPA, and the cell growth, cell apoptosis and expression of vascular endothelial growth factor (VEGF) and martix metalloproteinase-9 (MMP-9) protein were determined with MTT assay, flow cytometry and immunohistochemistry respectively. Results After treatment with VPA, the morphology of A2780 cells significantly changed, the cell growth was suppressed in a concentration- and time-dependent manner, and the expression of VEGF and MMP-9 protein decreased. Conclusion VPA may inhibit the growth, induce the apoptosis, and down-regulate the expression of VEGF and MMP-9 protein in human ovarian cancer A2780 cells, which indicates that VPA may contribute to the inhibition of tumor angiogenesis and metastasis.

Key words: ovarian neoplasms, valproic acid, vascular endothelial growth factor, martix metalloproteinase, cell apoptosis