上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2020, Vol. 40 ›› Issue (3): 326-.doi: 10.3969/j.issn.1674-8115.2020.03.008

• 论著·基础研究 • 上一篇    下一篇

低氘水联合富血小板血浆对大鼠糖尿病溃疡创面愈合的影响

王湘琦，赵超然，熊爱兵   

  1. 西南医科大学附属医院烧伤整形外科，泸州 646000
  • 出版日期:2020-03-28 发布日期:2020-04-09
  • 通讯作者: 熊爱兵，电子信箱：xiongyang22000@aliyun.com。
  • 作者简介:王湘琦（1994—），女，硕士生；电子信箱：672257635@qq.com。

Effect of deuterium depleted water combined with platelet-rich plasma on wound healing of diabetic ulcer in rats

WANG Xiang-qi, ZHAO Chao-ran, XIONG Ai-bing   

  1. Department of Burn and Plastic Surgery, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, China
  • Online:2020-03-28 Published:2020-04-09

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摘要： 目的·观察低氘水联合富血小板血浆对大鼠糖尿病溃疡创面愈合的影响，并探讨其可能的机制。方法·将100只雄性SD大鼠随机分为2组：正常对照组（A组，n20）及糖尿病组（n80）。糖尿病组大鼠经高脂饲料喂养4周后，采用腹腔注射链脲佐菌素（STZ）+皮肤全层切除复制大鼠糖尿病溃疡模型；随后将造模成功的72只大鼠随机分为糖尿病模型组（B组）、富血小板血浆组（C组）、低氘水组（D组）和低氘水联合富血小板血浆组（E组），每组各18只。A组普通饲料喂养4周后，腹腔注射等体积的柠檬酸-柠檬酸钠缓冲液+皮肤全层切除复制正常溃疡模型。各组分别予以对应处理并于处理3、7和14 d后处死动物，在各对应时间点测大鼠随机血糖；取创面及创缘组织，观察各组大鼠溃疡创面愈合情况、局部组织形态学变化，并采用酶联反应吸附法检测各组创面组织中基质金属蛋白酶-9（matrix metalloproteinase-9，MMP-9）、组织金属蛋白酶抑制物 -1（tissue inhibitor of metalloproteinase-1，TIMP-1）的含量。结果·D组及E组随机血糖较干预前降低。各糖尿病组创面炎症反应均较A组慢，E组肉芽成熟效果较B、C、D组好，在各干预组中效果最佳，新生血管及成纤维细胞出现时间早且数量多。A组TIMP-1含量明显高于各糖尿病组（PPPPP结论·低氘水对大鼠糖尿病溃疡创面愈合有促进作用；低氘水联合富血小板血浆对大鼠糖尿病溃疡创面愈合有显著促进作用，其可能与降低大鼠随机血糖、提高溃疡创面TIMP-1、抑制MMP-9表达有关。

关键词: 低氘水, 富血小板血浆, 糖尿病溃疡, 基质金属蛋白酶-9, 组织金属蛋白酶抑制物 -1, 大鼠

Abstract:

Objective · To observe the effect of deuterium depleted water combined with platelet-rich plasma on wound healing of diabetic ulcer in rats, and to explore its possible mechanism. Methods · Male SD rats were randomly divided into two groups, normal control group (group A, n20) and diabetic group (n80). Rats in the diabetic group were fed with high-fat diet for 4 weeks, and the rat diabetic ulcer model was replicatedintraperitoneal injection of streptozotocin (STZ) + skin full-thickness resection; then randomly divided into diabetic model group (group B), platelet-rich plasma group (group C), deuterium depleted water group (group D), and deuterium depleted water combined platelet-rich plasma group (group E), with 18 rats for each group. Group A with common feed was fed for 4 weeks after intraperitoneal injection of an equal volume of citric acid-sodium citrate buffer + skin full-thickness resection to replicate the normal ulcer model. The animals were sacrificed after treatment for 3, 7 and 14 d, and the random blood glucose was measured at each corresponding time point. The wound surface and wound margin tissue were taken to observe the wound healing and local histomorphology of each group. The contents of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the wound tissue of each group were detectedenzyme-linked reaction adsorption method. Results · Random blood glucose in group D and group E was lower than that before intervention. The inflammatory response of the wounds in each diabetic group was slower than that in group A. The granulation ripening effect of group E was faster than that of group B, C, and D. The effect was best in each intervention group, and the neovascularization and fibroblasts appeared earlier and in large quantities. The content of TIMP-1 in group A was significantly higher than that in group B, C, D and E (PPPPPConclusion · Deuterium depleted water can promote the healing of diabetic ulcer wounds. Deuterium depleted water combined with platelet-rich plasma can significantly promote the healing of diabetic ulcer wounds, which may be related to the decrease of random blood glucose, the increase of TIMP-1 and the inhibition of MMP-9 .

Key words: deuterium depleted water, platelet-rich plasma, diabetic ulcer, matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1, rat