上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2018, Vol. 38 ›› Issue (3): 276-.doi: 10.3969/j.issn.1674-8115.2018.03.007

• 论著（基础研究） • 上一篇    下一篇

胃泌素对大鼠激素性骨坏死的治疗作用

罗亚平 1 ，李莹莹 2 ，胡纯婷 2 ，王井龙 2 ，傅国辉 1, 2，陈诗慧 2   

  1. 上海交通大学 1. 附属第一人民医院病理中心，上海 200080；2. 基础医学院病理中心，细胞分化与凋亡教育部重点实验室，上海 200025
  • 出版日期:2018-03-28 发布日期:2018-05-03
  • 通讯作者: 陈诗慧，电子信箱：chensh2015@shsmu.edu.cn。
  • 作者简介:罗亚平（1989—），女，硕士生；电子信箱：2270461489@qq.com

Therapeutic effect of gastrin on steroid-associated osteonecrosis in rats

LUO Ya-ping1 , LI Ying-ying2 , HU Chun-ting2 , WANG Jing-long2 , FU Guo-hui1, 2, CHEN Shi-hui2   

  1. 1. Pathology Center, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China; 2. Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Pathology Center, Shanghai Jiao Tong University College of Basic Medical Sciences, Shanghai 200025, China
  • Online:2018-03-28 Published:2018-05-03

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摘要：  目的 · 探讨胃泌素对大鼠激素性骨坏死的治疗作用。方法 · 24 只 SD 大鼠随机分为正常对照组（正常组）、骨坏死对照组（骨坏死组）、骨坏死治疗组（治疗组）。骨坏死组和治疗组经尾静脉注射脂多糖 1 次 /d（600 μg/kg）×2 d，同时肌内注射甲基泼尼松龙 1 次 /d（50 mg/kg）×3 d；对照组注射等量生理盐水。甲基泼尼松龙末次注射后，治疗组腹腔注射胃泌素 1 次 /d（800 μg/kg） ×14 d，骨坏死组注射等量生理盐水。治疗结束后，大鼠处死取股骨头生长板下骨小梁，通过苏木精 - 伊红染色法（hematoxylin-eosin staining，H-E 染色法）、免疫组织化学法、荧光染色法、戈尔德染色法进行组织学分析和鉴定。结果 · 成功构建大鼠激素性骨坏死模型。H-E 染色结果显示，治疗组较骨坏死组血栓面积、脂肪细胞数量及面积显著减少（均 P<0.05）；免疫组织化学试验结果显示，治疗组较骨坏死组成骨相关转录因子（Sp7）阳性细胞显著增加（P<0.01）；戈尔德染色与荧光检测结果显示，治疗组较骨坏死组类骨质长度和面积明显增加（均 P<0.01），骨表面骨形成率和矿化沉积速率明显升高（均 P<0.01）。结论 · 胃泌素能够通过抑制骨髓中骨小梁内的血栓和脂肪形成、促进骨小梁的成骨来治疗大鼠激素性骨坏死。

关键词: 激素性骨坏死, 胃泌素, 骨组织形态计量学, 大鼠

Abstract:

Objective · To investigate therapeutic effect of gastrin on steroid-associated osteonecrosis (SAON) in rat model. Methods · Twenty-four SD rats were randomly divided into three groups i.e. normal control group (normal group), SAON control group (SAON group) and SAON treatment group (treatment group). SAON group and treatment group were intravenously injected with lipopolysaccharide 1 time per day (600 μg/kg) for 2 d and meanwhile intramuscularly injected with methylprednisolone 1 time per day (50 mg/kg) for 3 d. Normal group was injected with normal saline of the same volumns. After steroid injections, treatment group was injected with gastrin 1 time per day (800 μg/kg) for 14 d, while SAON group was injected with normal saline of the same volumns. After the treatment, bone trabeculas below femoral head growth plate were dissected in the rats for bone histology. Hematoxylin-eosin (H-E) staining, immunohistochemistry, fluorescence staining and Goldner's trichrome staining were applied in this study. Results · SAON model in rats was successfully established. The result of H-E staining showed that compared with SAON group, thrombus area, number and area of fat cells in the bone marrows of treatment group obviously decreased (all P<0.05). Immunohistochemistry showed that osteogenic transcription factor (Sp7) positive cells in treatment group were more than those in SAON group (P<0.01). Compared with SAON group, osteoid length and area (Goldner′s trichrome staining), and bone formation rate and bone mineralization deposition rate (fluorescence staining) all significantly increased in treatment group (all P<0.01). Conclusion · Gastrin can effectively treat SAON in rats by suppressing thrombus and lipid formation and enhancing boneformation.

Key words: steroid-associated osteonecrosis (SAON), gastrin, bone histomorphometry, rat