上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2018, Vol. 38 ›› Issue (3): 272-.doi: 10.3969/j.issn.1674-8115.2018.03.006

• 论著（基础研究） • 上一篇    下一篇

丹参注射液对大鼠脊髓损伤后脑源性神经营养因子及胰岛素样生长因子 -1 表达的影响

刘 杨，高玉亭，苗宇船   

  1. 山西中医药大学 基础医学院病理教研室，晋中 030619
  • 出版日期:2018-03-28 发布日期:2018-05-03
  • 通讯作者: 苗宇船，电子信箱：mych65@163.com。
  • 作者简介:刘 杨（1980—），男，副教授，博士；电子信箱：syfy2004@163.com。
  • 基金资助:
     山西中医药大学博士科研启动基金

Effect of Danshen injection on expression of brain-derived neurotrophic factor and insulin-like growth factor-1 in rats with spinal cord injury

LIU Yang, GAO Yu-ting, MIAO Yu-chuan   

  1. Department of Pathology, College of Basic Medicine, Shanxi University of Chinese Medicine, Jinzhong 030619, China
  • Online:2018-03-28 Published:2018-05-03
  • Supported by:
    Scientific Research Fund for Doctoral Young Scholars，Shanxi University of Chinese Medicine

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摘要： 目的 · 观察丹参注射液对大鼠脊髓损伤（spinal cord injury，SCI）后脑源性神经营养因子（brain-derived neurotrophic factor， BDNF）和胰岛素样生长因子 -1（insulin-like growth factor-1，IGF-1）表达的影响。方法 · SD 大鼠 30 只随机分为正常对照组、模型组和治疗组各 10 只，其中正常对照组常规饲养，模型组和治疗组的大鼠建立 Allens’重力打击 SCI 模型。按每日 1 mL/kg 剂量分别腹腔注射生理盐水和丹参注射液，连续给药 14 d。伤后 1、3、7 及 14 d 时采用联合行为评分法（combine behavioral score，CBS）评价大鼠脊髓神经功能。14 d 时采用免疫组织化学染色和 Western blotting 检测各实验组大鼠脊髓组织 BDNF 和 IGF-1 的表达。结果 · 与正常对照组比较，模型组和治疗组大鼠的 CBS 评分升高，并随损伤时间的延长出现不同程度的降低，至伤后 14 d，治疗组大鼠的 CBS 评分显著低于模型组（P=0.000）；与模型组比较，治疗组大鼠 BDNF 和 IGF-1 的阳性细胞数和相对表达量均显著升高（均 P<0.05）。结论 · 丹参注射液可通过调节 BDNF 和 IGF-1 的表达促进脊髓的损伤修复。

关键词: &ensp, 脊髓损伤；丹参注射液；脑源性神经营养因子；胰岛素样生长因子 -1

Abstract:

Objective · To explore the mechanism of Danshen injection on promoting spinal cord functional recovery by observing the expression of brain-derived neurotrophic factor (BDNF) and insulin-like growth factor-1 (IGF-1) in rats with spinal cord injury (SCI). Methods · Thirty SD rats were divided randomly into normal control group, model group and treatment group (n=10). The rats in model group and treatment group were subjected to weight-drop SCI according to the Allens' method, and administered normal saline and Danshen injection, at the dosage of 1 mL/kg once a day , by intraperitoneal injection respectively for 14 days. The combine behavioral score (CBS) of rats in all groups was detected on 1, 3, 7 and 14 days after SCI, the expression of BDNF and IGF-1 in all groups was detected with immunohistochemical method and Western blotting. Results · Compared with the normal control group, the CBS in model group and treatment group was elevated after SCI, and declined gradually with the lapse of injury time in different rates. Until 14 days after SCI, compared with the model group, the CBS in treatment group was lower (P=0.000), and the expression levels of BDNF and IGF-1 (including immunohistochemistry positive cell number and relative content) in treatment group were both elevated significantly (all P<0.05). Conclusion · Danshen injection can promote the recovery of nerve function by elevating the expression of BDNF and IGF-1 after SCI.

Key words: spinal cord injury (SCI), Danshen injection, brain-derived neurotrophic factor (BDNF), insulin-like growth factor-1 (IGF-1)