上海交通大学学报（医学版）

›› 2013, Vol. 33 ›› Issue (5): 607-.doi: 10.3969/j.issn.1674-8115.2013.05.020

• 论著（基础研究） • 上一篇    下一篇

灵芝多糖在Graves病小鼠模型中对甲亢及甲亢肝脏功能损伤的影响

赵泽飞1, 赵咏桔2, 顾明君1, 崔九兰3, 王 曙4   

  1. 1.上海市浦东新区公利医院内分泌代谢病科, 上海 200135； 2.上海交通大学 医学院附属瑞金医院老年病科, 上海 200025； 3.上海市内分泌代谢病研究所, 上海 200025； 4.上海交通大学 医学院附属瑞金医院内分泌代谢病科, 上海 200025
  • 出版日期:2013-05-28 发布日期:2013-05-28
  • 通讯作者: 王 曙, 电子信箱: shuwang999@hotmail.com。
  • 作者简介:赵泽飞（1977—）, 女, 博士, 主治医师； 电子信箱: zhaozefei@126.com。
  • 基金资助:

    上海市卫生局青年科研项目（2010Y054）；上海市浦东新区卫生局卫生科研项目（青年基金）（PW2010B-3）；上海市浦东新区卫生系统优秀青年医学人才项目(PWRq2010-02)

Effect of Ganoderma lucidum polysaccharides on hyperthyroidism and liver injury in Graves´|disease mice model

ZHAO Ze-fei1, ZHAO Yong-ju2, GU Ming-jun1, CUI Jiu-lan3, WANG Shu4   

  1. 1.Department of Endocrine and Metabolic Diseases, Gongli Hospital, Shanghai 200135, China； 2.Department of Geriatrics, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China； 3.Shanghai Institute of Endocrinology and Metabolism, Shanghai 200025, China； 4.Department of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
  • Online:2013-05-28 Published:2013-05-28
  • Supported by:

    Shanghai Municipal Health Bureau Foundation, 2010Y054；Shanghai Pudong New District Health Bureau Foundation, PW2010B-3； Shanghai Pudong New District Health System Excellent Young Medical Talent Project, PWRq2010-02

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摘要：

目的 探讨灵芝多糖对Graves病(GD)模型小鼠甲状腺功能亢进症（甲亢）及其所致肝脏功能损伤的影响。方法 利用促甲状腺素受体A亚单位（TSHR-A）免疫BALB/c小鼠获得GD动物模型，检测小鼠血清甲状腺素（T4）、促甲状腺素受体抗体（TRAb）和肝功能指标并分析相关性。在建模过程中，分别给予纯水、低剂量灵芝多糖（100 mg·kg-1·d-1）和高剂量灵芝多糖（400 mg·kg-1·d-1）灌胃，观察小鼠血清T4、TRAb和肝功能指标谷丙转氨酶(ALT)、谷草转氨酶（AST）和碱性磷酸酶（ALP）的变化。结果 与模型组未发生甲亢和对照组小鼠比较，模型组发生甲亢小鼠的血清ALT、AST和ALP水平均显著升高（P<0.05），血清AST、ALP与T4水平呈显著正相关（r值分别为0.585和0.744，P<0.05），与TRAb水平无相关性（P>0.05）。灵芝多糖干预后，模型小鼠血清T4和TRAb无显著变化（P>0.05）。经低剂量灵芝多糖干预的模型组发生甲亢小鼠的肝功能指标（血清ALT和ALP）均显著优于纯水组（P<0.05）。结论 灵芝多糖对GD模型小鼠的甲亢状态无明显影响，但对甲亢引起的肝脏功能损伤具有改善作用。

关键词: Graves病, 灵芝多糖, 甲状腺功能亢进症, 肝脏损伤

Abstract:

Objective To investigate the effect of Ganoderma lucidum polysaccharides on hyperthyroidism and liver injury in the mice model of Graves´ disease (GD). Methods GD mice model was constructed by immunizing BALB/c mice with thyroid stimulating hormone receptor-A (TSHR-A). Serum thyroxine (T4), thyroid stimulating hormone receptor antibody (TRAb), and liver function parameters were measured, and correlation analysis was performed. The mice were intragastrically managed with pure water, low-dose Ganoderma lucidum polysaccharides (100 mg·kg-1·d-1) and high-dose Ganoderma lucidum polysaccharides (400 mg·kg-1·d-1) respectively, and the change of serum T4, TRAb, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were determined. Results Serum ALT, AST and ALP in mice with hyperthyroidism in model group were significantly higher than those in mice without hyperthyroidism in model group and mice in control group (P<0.05). Serum AST and ALP were positively significantly related to serum T4(r=0.585, P<0.05; r=0.744, P<0.05), but were not related to serum TRAb (P>0.05). Serum T4 and TRAb in model mice were not significantly changed after treatment with Ganoderma lucidum polysaccharides (P>0.05). Serum ALT and ALP in mice with hyperthyroidism in model group treated with low-dose Ganoderma lucidum polysaccharides were significantly superior to those in pure water group (P<0.05). Conclusion Ganoderma lucidum polysaccharides may not work on hyperthyroidism improvement in GD mice model, but can improve the liver injury caused by hyperthyroidism.

Key words: Graves´, disease, Ganoderma lucidum polysaccharides, hyperthyroidism, liver injury