上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2013, Vol. 33 ›› Issue (6): 776-.doi: 10.3969/j.issn.1674-8115.2013.06.016

• 论著（基础研究） • 上一篇    下一篇

索拉菲尼对人肾癌裸鼠移植肿瘤生长的抑制作用

汪 磊，周佩军，徐 达，王祥慧，邵 琨   

  1. 上海交通大学 医学院瑞金医院泌尿外科， 上海 200025
  • 出版日期:2013-06-28 发布日期:2013-06-28
  • 通讯作者: 周佩军, 电子信箱: peijunzhou@yhaoo.com.cn。
  • 作者简介:汪磊(1985—)，男， 硕士生; 电子信箱: wangl850702@gmail.com。

Sorafenib inhibits growth of human renal cell carcinoma xenografted in nude mice

WANG Lei, ZHOU Pei-jun, XU Da, WANG Xiang-hui, SHAO Kun   

  1. Department of Urology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
  • Online:2013-06-28 Published:2013-06-28

PDF (PC)

1468

摘要：

目的 建立人肾癌裸鼠移植肿瘤模型，观察不同剂量索拉菲尼对移植肿瘤生长的抑制作用。方法 BALB/c裸鼠经皮下接种人肾透明细胞癌皮肤转移细胞（Caki-1）建立荷Caki-1裸鼠肿瘤模型。细胞接种1周后，采用小动物18氟-氟代脱氧葡萄糖正电子发射断层显像/X线计算机体层扫描（18F-FDG PET/CT）技术评价实体肿瘤模型。选取肿瘤生长情况接近的荷瘤裸鼠18只，随机分为对照组和索拉菲尼低、高剂量组（n=6），于细胞接种后第35天处死动物，测量各组荷瘤裸鼠的体质量及肿瘤体积和质量，观察移植肿瘤的组织学改变。另选取肿瘤生长情况接近的荷瘤裸鼠15只进行如上分组（n=5），预设观察期100 d，比较存活率。结果 荷瘤裸鼠肿瘤组织18F-FDG吸收代谢水平高于正常组织。与对照组比较，索拉菲尼低、高剂量组荷瘤裸鼠的肿瘤生长均被明显抑制，各组间肿瘤体积和质量比较差异均有统计学意义（P<0.05）。索拉菲尼高剂量组裸鼠的体质量显著低于对照组和索拉菲尼低剂量组（P<0.05）。索拉菲尼低、高剂量组荷瘤裸鼠的存活率显著高于对照组（P<0.05）。结论 小动物18F-FDG PET/CT可用于评价肾癌细胞在宿主体内的生长状况，索拉菲尼对人肾癌裸鼠皮下移植肿瘤模型中肾癌细胞的生长具有显著抑制作用。

关键词: 肾癌, 裸鼠, 索拉菲尼, 18氟-氟代脱氧葡萄糖正电子发射断层显像/X线计算机体层扫描, 小动物

Abstract:

Objective To establish the xenogenous implant model of human renal cell carcinoma in nude mice, and observe the inhibition effect of different doses of sorafenib on the growth of tumor. Methods Tumor-bearing nude mouse models were developed by subcutaneous implantation of Caki-1 cells into BALB/c mice, and were evaluated by small animal fluorine-18 fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) one week after implantation. Eighteen tumor-bearing mice were randomly divided into control group, low-dose sorafenib group and high-dose sorafenib group, with 6 mice in each group. Thirty-five days after Caki-1 implantation, mice were sacrificed, the body weight of mice and volumes and weight of tumors were measured, and the histological changes of tumors were observed. Another three similar groups were set up, with 5 mice in each group, and the survival of nude mice were compared in the expected observation duration of 100 d. Results The uptake of 18F-FDG in tumor tissues was significantly higher than that in normal tissues. Compared with control group, the tumor growth in lowdose sorafenib group and high-dose sorafenib group was significantly inhibited, and there were significant differences in the volumes and weight of tumors among groups (P<0.05). The body weight of nude mice in high-dose sorafenib group was significantly lower than that in control group and lowdose sorafenib group (P<0.05). The survival of nude mice in low-dose sorafenib group and high-dose sorafenib group was significantly higher than that in control group (P<0.05). Conclusion Small animal 18F-FDG PET/CT can be used in evaluation of growth of renal carcinoma cells in host, and sorafenib can significantly inhibit the growth of renal carcinoma cells in xenogenous implant model of human renal cell carcinoma in nude mice.

Key words: renal cell carcinoma, nude mice, sorafenib, fluorine-18 fluorodeoxyglucose positron emission tomography/computed tomography, small animal