上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

pro-1基因通过DAF-16调控秀丽隐杆线虫的寿命

毛玉琴1， 韩三峰2， 王立顺1   

  1. 1．上海交通大学 医学院附属瑞金医院分子医学中心， 上海 200025； 2.复旦大学附属闵行医院， 上海 201100
  • 出版日期:2016-10-28 发布日期:2016-11-29
  • 通讯作者: 王立顺， 电子信箱： jywangls@shsmu.edu.cn。
  • 作者简介:毛玉琴（1992—）， 女， 硕士生； 电子信箱： myq110813@163.com。
  • 基金资助:

    国家自然科学基金(81472758, 31170783)

Pro-1 regulates the lifespan of Caenorhabditis elegans through DAF-16

MAO Yu-qin1, HAN San-feng2, WANG Li-shun1   

  1. 1.Center for Molecular Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 2.Minhang Hospital, Fudan University, Shanghai 201100, China
  • Online:2016-10-28 Published:2016-11-29
  • Supported by:

    National Natural Science Foundation of China, 81472758, 31170783

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摘要：

目的·用秀丽隐杆线虫（Caenorhabditis elegans）作为模式生物，观察pro-1基因对寿命的影响，并初步探究其影响寿命的机制。方法·在线虫上以RNAi技术定向干扰pro-1基因的表达，随后对pro-1沉默表达后的线虫进行寿命分析，检测其在生长发育、运动、饮食以及生殖能力等方面的变化。进一步用RNAi方法结合线虫体内荧光定位技术以及RT-PCR技术等探究pro-1影响寿命可能的分子机制。结果·与对照组线虫相比，干扰 pro-1表达后，沉默组线虫平均寿命延长了14.58%(P=0.002)；在TJ356线虫中干扰pro-1表达后，DAF-16明显进入细胞核，且DAF-16下游靶基因如old-1、sod-3、hsp-16.1、mtl-1、ctl-2等的mRNA水平被上调。结论·pro-1基因通过促进DAF-16进入细胞核并上调其下游靶基因的mRNA水平，从而延长秀丽隐杆线虫的寿命。

关键词: 秀丽隐杆线虫, pro-1, daf-16, 寿命

Abstract:

Objective·To observe the effects of pro-1 gene on the lifespan of Caenorhabditis elegans (C. elegans) and preliminary explore the molecular mechanisms. Methods·RNAi technique was used to intervene the expression of pro-1 gene in C. elegans. The lifespan of C. elegans with silent pro-1 was analyzed and changes in growth, development, motion, diet, and reproduction were detected. The molecular mechanisms of pro-1 to affect the lifespan were explored using RNAi technique, fluorescence localization technology, and RT-PCR. Results·The lifespan of silence group C. elegans was significantly increased by 14.58% after the expression of pro-1  intervented as compared with controls (P=0.002). DAF-16 translocated into nucleus in C. elegans  of TJ356 strain after intervening the expression of pro-1 and mRNA expressions of downstream target genes such as old-1, sod-3, hsp-16.1, mtl-1 and ctl-2 were up-regulated. Conclusion·pro-1 prolongs the lifespan of C. elegans via promoting the translocation of DAF-16 into nucleus and up-regulating the mRNA expressions of downstream target genes.

Key words: Caenorhabditis elegans, pro-1, daf-16, lifespan