Objective · To observe the effects of ultrasound-guided Mammotome procedure on the treatment of breast lumps. Methods · 1 034 patients (1 347 mammary focuses) underwent ultrasound-guided Mammotome procedure in the Department of Breast Disease at the International Peace Maternity & Child Health Hospital affiliated to Shanghai Jiao Tong University School of Medicine in 2013. The effects of ultrasound-guided Mammotome procedure on the diagnosis and treatment of mammary lumps were evaluated via pathological examinations and follow-up visits. Results · All focuses were successfully resected. The diameters of lumps ranged between 0.8 and 2.4 cm with the mean time of 10 min (2-20 min) and the average resected tissue strip number of 15 for resecting a single lump and the skin wound length of 0.3-0.5 cm. There were no serious complications. Postoperative pathological examinations suggested 15 (1.11%) cases of breast cancer. Conclusion · Ultrasound-guided Mammotome procedure can completely resect benign breast tumors and facilitates the early diagnosis of malignant breast tumors with accurate biopsy findings, small wound, short operation duration, and less postoperative complications.