上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2017, Vol. 37 ›› Issue (8): 1079-.doi: 10.3969/j.issn.1674-8115.2017.08.006

• 论著（基础研究） • 上一篇    下一篇

超高效液相色谱- 四极杆飞行时间质谱联用技术分析人类唾液代谢组的性别依赖性

李晶1, 2，刘思1, 2，雷和花1，王玉兰1#，唐惠儒3#   

  1. 1. 波谱与原子分子物理国家重点实验室，武汉磁共振中心，中国科学院 武汉物理与数学研究所，武汉 430071；2. 中国科学院大学，北京 100049；3. 复旦大学 生命科学学院，上海200438
  • 出版日期:2017-08-28 发布日期:2017-09-28
  • 作者简介:李晶（1991—），女，硕士生；电子信箱：lijing_sunny@outlook.com

Gender dependence of metabolomic phenotypes for human saliva using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry

LI Jing1, 2, LIU Si1, 2, LEI He-hua1, WANG Yu-lan1#, TANG Hui-ru3#   

  1. 1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China; 3. School of Life Sciences, Fudan University, Shanghai 200438, China
  • Online:2017-08-28 Published:2017-09-28

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摘要： 目的 · 研究人类唾液代谢组的性别依赖性。方法 · 使用基于超高效液相色谱 – 四极杆飞行时间质谱（UHPLC-Q-TOFMS）联 用的代谢组学技术，检测分析年龄、体质量指数、生活及洁牙条件匹配的 5 位男性与 5 位女性健康志愿者的唾液样本代谢物组成，再 通过与已有数据库对比和标准品验证鉴定唾液中的代谢物群，确定 2 组样品中差异具有统计学意义的代谢物。结果 · 鉴定了人类唾液 中的13 种氨基酸、6 种胆碱代谢物、15 种肉碱类代谢物、4 种鞘氨醇类代谢物、7 种溶血磷脂酰胆碱和3 种有机酸等48 种代谢物。 发现苯丙氨酸、乙酰基肉碱、丙酰基肉碱、丁酰基肉碱、异丁酰基肉碱、异戊酰基肉碱和鞘氨醇的含量在女性唾液中显著高于男性样 本。结论 · 人类唾液中含有丰富的代谢物信息，唾液代谢组研究必须考虑其性别依赖性。

关键词: 唾液, 代谢组学, 超高效液相色谱&ndash, 四极杆飞行时间质谱, 性别依赖

Abstract:

Objective · To investigate the gender-related metabolomic differences in human saliva.  Methods · The saliva metabolomic profiles of 5 male and 5 female healthy volunteers with matched age, body mass index (BMI), living and tooth-brushing condition were acquired using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry. Metabolites were identified using publicly accessible databases and further confirmed individually with standard compounds. Both multivariant and univariate statistics were conducted to find inter-gender differences.  Results · Forty-eight metabolites in human saliva were identified including 13 amino acids, 6 choline metabolites, 15 carnitines, 4 sphinganine metabolites, 7 lysophosphatidylcholine and 3 organic acids. Levels of phenylalanine, acetylcarnitine, propionylcarnitine, butyrylcarnitine, isobutyrylcarnitine, isovalerylcarnitine and sphinganine were higher in the saliva of females than that of males.  Conclusion · Rich metabolic information present in human saliva with significant gender diffference which ought to be taken into consideration in study on the metabonomics of saliva.

Key words: saliva, metabolomics, ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry, gender dependence