上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2019, Vol. 39 ›› Issue (11): 1239-.doi: 10.3969/j.issn.1674-8115.2019.11.004

• 论著·基础研究 • 上一篇    下一篇

大鼠深Ⅱ度灼伤后皮肤力学性质改变及变性真皮再利用的初步观察

王志勇1，王西樵2，刘英开2，董叫云2，宋 菲2，曹晓赞2，陆树良2   

  1. 1. 上海交通大学医学院附属瑞金医院灼伤整形科，上海 200025；2. 上海市烧伤研究所，上海 200025
  • 出版日期:2019-11-28 发布日期:2019-12-16
  • 通讯作者: 王西樵，电子信箱：wxqiao2002@hotmail.com。
  • 作者简介:王志勇（1971—），男，副主任医师，博士；电子信箱：wzy10830@rjh.com.cn。
  • 基金资助:
    国家自然科学基金（81671914，81071567，30872686，81000838）

Changes of skin mechanical properties and reof denatured dermis after deep second-degree burn in rats

WANG Zhi-yong1, WANG Xi-qiao2, LIU Ying-kai2, DONG Jiao-Yun2, SONG Fei2, CAO Xiao-zan2, LU Shu-liang2   

  1. 1. Department of Burns and Plastic Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025; 2. Shanghai Burns Institute, Shanghai 200025
  • Online:2019-11-28 Published:2019-12-16
  • Supported by:
    National Natural Science Foundation of China, 81671914, 81071567, 30872686, 81000838

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摘要： 目的·研究大鼠深Ⅱ度灼伤后皮肤力学性质的改变及变性真皮脱细胞后用于复合移植再利用的可行性。方法·建立SD大鼠背部深Ⅱ度灼伤模型，灼伤后3 h及3 d后切取皮肤组织，利用力学检测器，检测皮肤弹性模量及最大载荷；另外，制作正常皮肤及灼伤后3 h皮肤的脱细胞真皮基质，比较真皮基质的结构差异以及进行异体复合移植，观察移植成活皮片的大体及组织学结构。结果·大鼠正常皮肤、灼伤后3 h及3 d皮肤的弹性模量分别是（8.7±6.4） MPa、（7.2±2.7） MPa和（2.7±0.3） MPa，最大载荷分别是（107.1±41.7） N、（81.5±10.8） N和（42.2±7.0） N；灼伤后3 d皮肤的弹性模量和最大载荷均较正常组显著降低（均P结论·深Ⅱ度灼伤早期变性皮肤组织与正常皮肤的力学性质相近，组织结构相似，可作为脱细胞真皮基质的潜在来源进行复合移植。

关键词: 灼伤, 变性真皮, 生物力学, 脱细胞真皮基质, 复合移植

Abstract:

Objective · To investigate the changes of skin mechanical properties after deep second-degree burn, and study the feasibility of composite transplantationusing denatured dermal acellular matrix. Methods · The deep second-degree burn models in SD rats were constructed, and then the full skins were harvested after 3 h and 3 d, respectively, to compare the skin modulus of elasticity and ultimate load with normal skins. In addition, the structural differences of the dermal matrix were also compared. The composite transplantations were conductedusing the normal or denatured dermal acellular matrix, and then the general and histological structures of living skin grafts were observed. Results · The moduli of elasticity of the normal skins and the skins 3 h and 3 d after burn in SD rats were (8.7±6.4) MPa, (7.2±2.7) MPa and (2.7±0.3) MPa, respectively. The ultimate loads were (107.1±41.7) N, (81.5±10.8) N and (42.2±7.0) N, respectively. Both indexes were significantly reduced in the skins 3 d after burn compared to the normal ones (PConclusion · The early denatured skin tissue after deep second-degree burn maintains the similar mechanical properties and histological structure to the normal skin, which is a potential source of acellular dermal matrix for composite transplantation.

Key words: burn, denatured dermis, biomechanics, acellular dermal matrix, composite transplantation

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