原位生物打印的研究进展与前景

doi:10.3969/j.issn.1674-8115.2021.02.016

上海交通大学学报(医学版) ›› 2021, Vol. 41 ›› Issue (2): 228-232.doi: 10.3969/j.issn.1674-8115.2021.02.016

原位生物打印的研究进展与前景

李文韬(), 王金武()

上海交通大学医学院附属第九人民医院骨科，上海 200011

收稿日期:2020-02-26 出版日期:2021-02-28 发布日期:2021-02-28
通讯作者: 王金武 E-mail:li_wentao@sjtu.edu.cn;Jinwu_wang@163.com
作者简介:李文韬（1996—），男，硕士生；电子信箱：li_wentao@sjtu.edu.cn。
基金资助:
国家重点研发计划(2018YFB1105600);国家自然科学基金(81572156);上海市科学技术委员会项目(19XD1434200);上海市教育委员会高峰高原学科建设计划(20152224)

Progress and prospect of in situ bioprinting

Wen-tao LI(), Jin-wu WANG()

Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China

Received:2020-02-26 Online:2021-02-28 Published:2021-02-28
Contact: Jin-wu WANG E-mail:li_wentao@sjtu.edu.cn;Jinwu_wang@163.com
Supported by:
National Key Research and Development Program of China(2018YFB1105600);National Natural Science Foundation of China(81572156);Project of Science and Technology Commission of Shanghai Municipality(19XD1434200);Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Grant Support(20152224)

摘要/Abstract

摘要：

生物打印是一种新兴的生物制造技术，推动了许多创新并为再生医疗开辟了新途径。生物打印的目的是在体外制造移植物，然后将其植入体内，然而一直以来离体组织的培养及血管化都存在难以彻底解决的问题。为了解决这一难题，研究者们提出了原位生物打印的概念，将组织直接打印在受伤或缺损的部位，利用体内的天然细胞微环境使打印组织成熟。该文对原位生物打印的技术、优势、应用领域、发展方向等进行综述。

关键词: 原位生物打印, 组织再生, 生物墨水

Abstract:

Bioprinting is a new biological manufacturing technology, which opens up new ways for regenerative therapy. The purpose of bioprinting is to create the internal plants in vitro. However, it is difficult to culture and vascularize printed tissue in vitro. In order to overcome this difficulty, in situ printing is presented to print the tissue directly on the injured or defective site, using the natural microenvironment in vivo to make the printed tissue mature. This article reviews the technology, advantages and research status of in situ bioprinting and briefly introduces the future direction of in situ bioprinting.

Key words: in situ bioprinting, tissue regeneration, bioink

中图分类号:

Q819

李文韬, 王金武. 原位生物打印的研究进展与前景[J]. 上海交通大学学报(医学版), 2021, 41(2): 228-232.

Wen-tao LI, Jin-wu WANG. Progress and prospect of in situ bioprinting[J]. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), 2021, 41(2): 228-232.

参考文献 37

1	Murphy SV, Atala A. 3D bioprinting of tissues and organs [J]. Nat Biotechnol, 2014, 32(8): 773-785.
2	Zhang YS, Yue K, Aleman J, et al. 3D bioprinting for tissue and organ fabrication[J]. Ann Biomed Eng, 2017, 45(1): 148-163.
3	Ozbolat IT. Bioprinting scale-up tissue and organ constructs for transplantation[J]. Trends Biotechnol, 2015, 33(7): 395-400.
4	Campbell PG, Weiss LE. Tissue engineering with the aid of inkjet printers[J]. Expert Opin Biol Ther, 2007, 7(8): 1123-1127.
5	Ashammakhi N, Ahadian S, Pountos I, et al. In situ three-dimensional printing for reparative and regenerative therapy[J]. Biomed Microdevices, 2019, 21(2): 42.
6	Wang MY, He JK, Liu YX, et al. The trend towards in vivo bioprinting[J]. Int J Bioprinting, 2015, 1(1): 15-26.
7	Singh S, Choudhury D, Yu F, et al. In situ-bioprinting:bioprinting from benchside to bedside?[J]. Acta Biomater, 2020, 101: 14-25.
8	Gudapati H, Dey M, Ozbolat I. A comprehensive review on droplet-based bioprinting: past, present and future[J]. Biomaterials, 2016, 102: 20-42.
9	Ozbolat IT, Hospodiuk M. Current advances and future perspectives in extrusion-based bioprinting[J]. Biomaterials, 2016, 76: 321-343.
10	Sorkio A, Koch L, Koivusalo L, et al. Human stem cell based corneal tissue mimicking structures using laser-assisted 3D bioprinting and functional bioinks[J]. Biomaterials, 2018, 171: 57-71.
11	Keriquel V, Guillemot F, Arnault I, et al. In vivo bioprinting for computer-and robotic-assisted medical intervention: preliminary study in mice[J]. Biofabrication, 2010, 2(1): 014101.
12	Cohen DL, Lipton JI, Bonassar LJ, et al. Additive manufacturing for in situ repair of osteochondral defects[J]. Biofabrication, 2010, 2(3): 035004.
13	Keriquel V, Oliveira H, Rémy M, et al. In situ printing of mesenchymal stromal cells, by laser-assisted bioprinting, for in vivo bone regeneration applications[J]. Sci Rep, 2017, 7(1): 1778.
14	Albanna M, Binder KW, Murphy SV, et al. In situ bioprinting of autologous skin cells accelerates wound healing of extensive excisional full-thickness wounds[J]. Sci Rep, 2019, 9(1): 1856.
15	Lipskas J, Deep K, Yao W. Robotic-assisted 3D bio-printing for repairing bone and cartilage defects through a minimally invasive approach[J]. Sci Rep, 2019, 9(1): 3746.
16	Ding HZ, Chang RC. Simulating image-guided in situ bioprinting of a skin graft onto a phantom burn wound bed[J]. Addit Manuf, 2018, 22: 708-719.
17	O'Connell CD, Di Bella C, Thompson F, et al. Development of the Biopen: a handheld device for surgical printing of adipose stem cells at a chondral wound site[J]. Biofabrication, 2016, 8(1): 015019.
18	Murdock MH, Badylak SF. Biomaterials-based in situ tissue engineering[J]. Curr Opin Biomed Eng, 2017, 1: 4-7.
19	Hakimi N, Cheng R, Leng L, et al. Handheld skin printer: in situ formation of planar biomaterials and tissues[J]. Lab Chip, 2018, 18(10): 1440-1451.
20	Binder KW, Zhao WX, Aboushwareb T, et al. In situ bioprinting of the skin for burns[J]. J Am Coll Surg, 2010, 211(3): S76.
21	Sofokleous P, Stride E, Bonfield W, et al. Design, construction and performance of a portable handheld electrohydrodynamic multi-needle spray gun for biomedical applications[J]. Mater Sci Eng C Mater Biol Appl, 2013, 33(1): 213-223.
22	Makris EA, Gomoll AH, Malizos KN, et al. Repair and tissue engineering techniques for articular cartilage[J]. Nat Rev Rheumatol, 2015, 11(1): 21-34.
23	Duchi S, Onofrillo C, O'Connell CD, et al. Handheld co-axial bioprinting: application to in situ surgical cartilage repair[J]. Sci Rep, 2017, 7(1): 5837.
24	Di Bella C, Duchi S, O'Connell CD, et al. In situ handheld three-dimensional bioprinting for cartilage regeneration[J]. J Tissue Eng Regen Med, 2018, 12(3): 611-621.
25	Duchi S, Onofrillo C, O'Connell CD, et al. Innovative cartilage regeneration for in situ co-axial 3D bioprinting [J]. Tissue Engineering Part A, 2017, 23: S22.
26	Ibrahim A. 3D bioprinting bone[M]//Thomas DJ.3D bioprinting for reconstructive surgery.Duxford: Elsevier, 2018: 245-275.
27	Kérourédan O, Hakobyan D, Rémy M, et al. In situ prevascularization designed by laser-assisted bioprinting: effect on bone regeneration[J]. Biofabrication, 2019, 11(4): 045002.
28	Li L, Yu F, Shi JP, et al. In situ repair of bone and cartilage defects using 3D scanning and 3D printing[J]. Sci Rep, 2017, 7(1): 9416.
29	Jin C, Zhang J, Li XK, et al. Injectable 3-D fabrication of medical electronics at the target biological tissues[J]. Sci Rep, 2013, 3: 3442.
30	Sun XY, Yuan B, Rao W, et al. Amorphous liquid metal electrodes enabled conformable electrochemical therapy of tumors[J]. Biomaterials, 2017, 146: 156-167.
31	Li C, Faulkner-Jones A, Dun AR, et al. Rapid formation of a supramolecular polypeptide-DNA hydrogel for in situ three-dimensional multilayer bioprinting[J]. Angew Chem Int Ed Engl, 2015, 54(13): 3957-3961.
32	Li YC, Zhang YS, Akpek A, et al. 4D bioprinting: the next-generation technology for biofabrication enabled by stimuli-responsive materials[J]. Biofabrication, 2016, 9(1): 012001.
33	Ashammakhi N, Ahadian S, Fan ZJ, et al. Advances and future perspectives in 4D bioprinting[J]. Biotechnol J, 2018, 13(12): e1800148.
34	Kahn JS, Hu YW, Willner I. Stimuli-responsive DNA-based hydrogels: from basic principles to applications[J]. Acc Chem Res, 2017, 50(4): 680-690.
35	Ozbolat IT, Moncal KK, Gudapati H. Evaluation of bioprinter technologies[J]. Addit Manuf, 2017, 13: 179-200.
36	Zhu ZJ, Guo SZ, Hirdler T, et al. 3D printing: 3D printed functional and biological materials on moving freeform surfaces (adv. Mater. 23/2018)[J]. Adv Mater, 2018, 30(23): 1870165.
37	Evans CH. Gene delivery to bone[J]. Adv Drug Deliv Rev, 2012, 64(12): 1331-1340.

原位生物打印的研究进展与前景

Progress and prospect of in situ bioprinting

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献 37

相关文章 1

编辑推荐

Metrics