上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

小青春期的抑制对雄性大鼠性发育的影响

王瑞芳，董治亚，王 伟，肖 园，倪继红，王德芬   

  1. 上海交通大学 医学院附属瑞金医院儿内科， 上海 200025
  • 出版日期:2013-11-28 发布日期:2013-12-03
  • 通讯作者: 董治亚, 电子信箱: dzy831@126.com.cn。
  • 作者简介:王瑞芳（1987—）， 女， 硕士; 电子信箱: wangruifang1987@126.com。

Influence of hormone inhibition during minipuberty on sex development of male rats

WANG Rui-fang, DONG Zhi-ya, WANG Wei, XIAO Yuan, NI Ji-hong, WANG De-fen   

  1. Department of Pediatrics, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
  • Online:2013-11-28 Published:2013-12-03

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摘要：

目的 观察小青春期激素水平的抑制对雄性大鼠青春期及成年期性腺轴功能的影响。方法 ①观察正常雄性大鼠出生后不同时间点血清睾酮（T）、促黄体生成素（LH）、卵泡刺激素（FSH）浓度和双侧睾丸质量变化，以确立小青春期的存在；②对新生雄性大鼠出生后5 min内即予乙醚吸入以建立“无小青春期”模型；③比较45和75 d龄造模组和对照组大鼠双侧睾丸和附睾质量及睾丸组织的病理变化，采用ELISA法测血清T浓度，并以Real-Time PCR方法比较睾丸雄激素受体（AR）、胰岛素样因子3（INSL3）、抗苗氏管激素（AMH）和Ghrelin基因表达量在两组间的差异。结果 ①雄性大鼠血清T、LH浓度均在出生后2 h达短暂的高峰，而FSH出生后30 min内即出现高峰值，双侧睾丸质量也在出生后4 h出现短暂的高峰；②造模组大鼠出生后2 h血清T、LH和FSH均较对照组明显降低，成功建立了“无小青春期”模型；③45 d龄造模组睾丸病理切片见受损表现，45 d龄时ghrelin基因和75 d龄时AR基因mRNA表达两组比较差异无统计学意义，但造模组较对照组有明显的下降趋势（P=0.055和P=0.050）；75 d龄时造模组AMH基因mRNA的表达较对照组显著降低，差异有统计学意义（P=0.024）。结论 雄性大鼠亦存在出生后持续约6 h的小青春期；乙醚吸入抑制其小青春期后，可对大鼠青春期和性成熟期的性腺功能造成不同程度的影响。

关键词: 小青春期, 雄性大鼠, 性发育

Abstract:

Objective To investigate the impacts of hormone inhibition during minipuberty on adolescent gonadal axis function of male rats. Methods ①The concentrations of serum testosterone (T), luteotropic hormone (LH), follicle-stimulating hormone (FSH) and testicular weight of normal male rats were measured at different time points after birth (0, 2, 4, 6, and 24 h) to confirm the existence of minipuberty. ②“No minipuberty” models were established by using ether inhalation to male neonatal rats in 5 min after birth. ③Bilateral testes and epididymis weight and testicular pathologic changes were compared between the model and control group at the age of 45 d and 75 d. Serum T levels were tested by ELISA. And expressions of testicular androgen receptor (AR) mRNA, insulin-like growth factor 3 (INSL3) mRNA, anti-mullerian homone (AMH) mRNA, and Ghrelin mRNA were determined by real-time PCR assay. Results ①In the neonatal male rats, serum T and LH levels reached a maximum at 2 h after birth. FSH level rose to peak in 30 min, and bilateral testicular weight also reached a short-term peak at 4 h. ②In model group, serum T, LH, and FSH levels showed remarkable descent, which was proved establishment of “no minipuberty” model. ③Impaired evidences were found in testicular pathologic images of model group at the age of 45 d. Ghrelin gene mRNA expression at 45d of age and AR gene mRNA expression at 75 d of age had no statistical difference between the two group, but there was an obvious decrease tendency in model group (P=0.055, P=0.050). AMH mRNA expression in model group was significantly lower than that in the control group at 75 d of age (P=0.024). Conclusion Minipuberty was confirmed to exist in male rats which lasted about 6 h. Inhibition of minipuberty by ether inhalation was proved to have long-term effect on gonadal function of male rats.

Key words: minipuberty, male rat, sex development