上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

负载优化配比血小板源性生长因子-BB和胰岛素样生长因子-1纳米微球脱细胞人工真皮用于小鼠皮肤创面修复的研究

刘 诚，熊清华，付劲松，刘淑琴，熊 龙   

  1. 江西省人民医院整形外科， 南昌 330006
  • 出版日期:2014-11-28 发布日期:2014-12-02
  • 作者简介:刘 诚（1967—）， 男， 副主任医师， 硕士; 电子信箱: lc.67@126.com
  • 基金资助:

    江西省自然科学基金（20122BAB205008）

Study on acellular artificial dermis loaded with nanoparticles containing optimized ratio of platelet-derived growth factor-BB and insulin-like growth factor-1 for repairing skin wounds of mice

LIU Cheng, XIONG Qing-hua, FU Jin-song, LIU Shu-qin, XIONG Long   

  1. Department of Plastic Surgery, Jiangxi Provinicial People's Hospital, Nanchang 330006, China
  • Online:2014-11-28 Published:2014-12-02
  • Supported by:

    Natural Science Foundation of Jiangxi Province, 20122BAB205008

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摘要：

目的 构建负载不同配比血小板源性生长因子-BB (PDGF-BB)和胰岛素样生长因子-1 (IGF-1)纳米微球的脱细胞人工真皮，用于裸鼠皮肤缺损的修复，探索PDGF-BB和IGF-1联合用于创面修复的最佳配比。方法 用聚乳酸-聚羟基乙酸共聚物(PLGA)包被不同配比的PDGF-BB和IGF-1制成纳米缓释微球,并使纳米微球结合于脱细胞人工真皮。将负载纳米微球的脱细胞人工真皮移植至裸鼠创面动物模型中，通过记录创面愈合速度和愈合时间，检测局部组织中α-平滑肌肌动蛋白（α-SMA）和白介素-2 （IL-2）的蛋白表达，观察PDGF-BB和IGF-1不同质量配比（1∶4、1∶2、1∶1、2∶1、4∶1）组（n=5）的纳米微球脱细胞人工真皮对小鼠皮肤创面修复的作用效果，另设空白对照组。结果 当纳米微球脱细胞人工真皮负载PDGF-BB和IGF-1的质量配比为2∶1时，小鼠皮肤修复创面愈合速度较其他配比组及空白对照组更快（P<0.05），愈合时间更短（P<0.05），局部组织中α-SMA和IL-2蛋白表达量更高（P<0.05）。结论 脱细胞人工真皮结合含PDGF-BB和IGF-1的纳米微球用于创面修复时，PDGF-BB和IGF-1的最佳配比为2∶1。

关键词: 人工真皮, 血小板源性生长因子-BB, 胰岛素样生长因子-1, 纳米微球

Abstract:

Objective To construct the acellular artificial dermis loaded with nanoparticles that contain different ratios of platelet-derived growth factor-BB (PDGF-BB) and insulin-like growth factor-1 (IGF-1) for repairing skin wounds of nude mice and to explore the best ratio of PDGF-BB and IGF-1 for repairing skin wounds. Methods Sustained released nanoparticles were made by enveloping different ratios of PDGF-BB and IGF-1 with polylactic acid-glycolic acid (PLGA) and then were combined with acellular artificial dermis. Nanoparticle loaded acellular artificial dermis was transplanted to the wound model of nude mice. Effects of nanoparticle loaded acellular artificial dermis of groups with different mass ratio of PDGF-BB and IGF-1 (1∶4, 1∶2, 1∶1, 2∶1, and 4∶1)(n=5) on healing mice skin wounds were observed by recording the wound healing speed and the average healing time and by detecting the protein expressions of α-SMA and IL-2 in local tissues. In addition, the blank control group was also established. Results When the mass ratio of PDGF-BB and IGF-1 of nanoparticle loaded acellular artificial dermis was 2:1, the wound healing speed was faster than that of the blank control group and groups with other mass ratios (P<0.05); the average healing time was shorter (P<0.05); and the protein expressions of α-SMA and IL-2 in local tissues were higher (P<0.05). Conclusion When acellular artificial dermis loaded with nanoparticles that contain PDGF-BB and IGF-1 is used for skin wound healing, the best ratio of PDGF-BB and IGF-1 is 2∶1.

Key words: acellular artificial dermis, platelet-derived growth factor-BB, insulin-like growth factor-1, nanoparticle