上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

两种草酸钙结石大鼠模型的比较研究

巴元明1，曹秋实2   

  1. 1.湖北省中医院， 武汉 430061； 2.湖北中医药大学， 武汉 430061
  • 出版日期:2014-11-28 发布日期:2014-12-02
  • 作者简介:巴元明（1961—）， 男， 主任医师， 教授，硕士， 博士生导师; 电子信箱: 530707198@qq.com。
  • 基金资助:

    湖北省卫计委科研重点项目(JX6A09)；湖北省医学领军人才培养工程专项经费资助项目

Comparative study on two calcium oxalate calculus models of rats

BA Yuan-ming1, CAO Qiu-shi2   

  1. 1.Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan 430061, China; 2.Hubei University of Traditional Chinese Medicine, Wuhan 430061, China
  • Online:2014-11-28 Published:2014-12-02
  • Supported by:

    Key Research Project of Health and Family Planning Commission of Hubei Province, JX6A09; Hubei Province's Outstanding Medical Academic Leader Program

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摘要：

目的 比较乙二醇法与草酸铵法大鼠草酸钙结石模型的肾脏病理改变及功能损害情况，探讨两种草酸钙结石模型的选择与应用。方法 36只SPF级雄性健康Wistar大鼠随机分为空白对照组、乙二醇组和草酸铵组(n=12)。乙二醇组大鼠给予1%乙二醇自由饮水和每日2%氯化铵2 mL灌胃，草酸铵组大鼠给予5%草酸铵饲料。造模后，检测各组大鼠血尿素氮(UN)、血清肌酐(Cr)及血清钙(Ca2+)、磷(P)和镁(Mg2+)含量，24 h尿草酸、Ca2+、P和Mg2+含量；显微镜下观察肾组织切片中草酸钙结晶沉积及病理变化。结果 乙二醇组和草酸铵组大鼠的尿草酸、Ca2+及P含量明显高于空白对照组（P<0.05，P<0.01）。两种方法复制大鼠草酸钙结石模型的成石效果均较显著、稳定，但大鼠肾脏病理改变及功能损害程度的差异有统计学意义(P<0.05)。结论 与乙二醇法造模比较，草酸铵法造模致大鼠肾脏病理改变及肾损害较严重，更适合应用于结石病致肾功能受损的研究。

关键词: 草酸钙, 肾结石, 动物模型, 大鼠

Abstract:

Objective To compare the renal pathological changes and functional impairments of rats of the calcium oxalate calculus model established by the ethylene glycol (EG) method and ammonium oxalate (AmOx) method and to explore the selection and application of two calcium oxalate calculus models. Methods A total of 36 SPF level healthy male Wistar rats were randomly divided into the control group, EG group, and AmOx group (n=12). Each rat of the EG group was intragastrically administered 2% ammonium chloride(AC) of 2 mL per day and was administered 1% EG for free drink. Rats of the AmOx group were fed by forage with 5% AmOx. After models were established, levels of serum urea nitrogen, creatinine, calcium, phosphorus, magnesium, 24 h urinary oxalate were detected. The paraffin kidney sections were observed under the microscope for calcium oxalate deposit and pathological changes. Results The levels of urinary oxalate, calcium, and phosphorus of the EG group and AmOx group were significantly higher than those of the control group (P<0.05, P<0.01). Effects of both calcium oxalate calculus models established by two methods were remarkable and stable, whereas renal pathological changes and functional impairments of rats were significantly different (P<0.05). Conclusion Compared to the model established by EG method, the model established by AmOx method can cause severer renal pathological changes and functional impairment of rats and is more suitable for studying renal functional impairment due to nephrolithiasis.

Key words: calcium oxalate, renal calculus, animal model, rat