上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

微RNA-375在三阴性乳腺癌中的表达及生物学效应

杨 晨1，虞 佩2，李济宇3，楼谷音4，奚文崎1   

  1. 1.上海交通大学 医学院附属瑞金医院肿瘤科, 上海 200025; 2.上海交通大学 医学院附属瑞金医院外科， 上海 200025； 3.山东大学附属山东省立医院乳腺甲状腺外科， 济南 250021； 4.上海交通大学 医学院附属瑞金医院放疗科， 上海 200025
  • 出版日期:2014-11-28 发布日期:2014-12-02
  • 通讯作者: 奚文崎, 电子信箱: xwq11061@rjh.com.cn。
  • 作者简介:杨 晨（1981—）， 男， 住院医师， 博士; 电子信箱: natoyang@126.com。
  • 基金资助:

    上海高校青年教师培养资助计划(2012-046)

Expression and biologic effects of microRNA-375 in triple negative breast cancer

YANG Chen1, YU Pei2, LI Ji-yu3, LOU Gu-yin4, XI Wen-qi1   

  1. 1.Department of Medical Oncology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 2.Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 3.Department of Breast and Thyroid Surgery, Provincial Hospital Affilliated to Shandong University, Jinan 250021, China; 4.Department of Chemistry and Radiation Oncology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Online:2014-11-28 Published:2014-12-02
  • Supported by:

    Cultivation Program for Young Teachers in Shanghai Colleges, 2012-046

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摘要：

目的 探讨微RNA-375 (miRNA-375) 在三阴性乳腺癌中的表达及生物学效应。方法 采用Real-time PCR技术检测62例三阴性乳腺癌患者的癌组织及其癌旁正常组织中miRNA-375的表达，分析miRNA-375表达与临床病理学特征的关系。对乳腺癌细胞株MDA-MB-231和乳腺纤维腺瘤细胞株MCF-10A中miRNA-375的表达进行检测；将miRNA-375前体转染MDA-MB-231，CCK-8法和Transwell实验检测细胞增殖和侵袭力的变化。结果 三阴性乳腺癌组织中miRNA-375表达显著低于癌旁正常组织(P<0.001)，miRNA-375的表达与肿瘤的大小和是否存在腋窝淋巴结转移有关(P<0.001)。与MCF-10A比较，MDA-MB-231中miRNA-375的表达显著降低（P=0.021）。miRNA-375前体转染后，MDA-MB-231内miRNA-375表达显著上调，细胞增殖能力和侵袭力减弱。结论 三阴性乳腺癌组织和MDA-MB-231中miRNA-375的表达下调。miRNA-375对三阴性乳腺癌发生和发展具有一定的抑制作用。

关键词: 三阴性乳腺癌, 微RNA-375, 侵袭力

Abstract:

Objective To investigate the expression and biologic effects of microRNA-375 (miRNA-375) of triple negative breast cancer (TNBC). Methods The expression of miRNA-375 of tumor tissue and adjacent normal tissues of 62 patients with TNBC was detected by the Real-time PCR. The relationship of the expression of miRNA-375 and clinicopathological features was analyzed. The expressions of miRNA-375 in breast cancer cell line MDA-MB-231 and breast fibroadenoma cell line MCF-10A were detected. The miRNA-375 precursor molecule was transfected to MDA-MB-231. The variations of the proliferation and invasion of MDA-MB-231 were detected by CCK-8 kit and Transwell test. Results The expression of miRNA-375 of TNBC tissue was significantly lower than that of adjacent normal tissue (P<0.001) and was relevant to the tumor size and axillary lymph node metastasis (P<0.001). Compared to MCF-10A, the expression of miRNA-375 in MDA-MB-231 significantly decreased (P=0.021). After being transfected by the miRNA-375 precursor, the expression of miRNA-375 in MDA-MB-231 significantly increased and the proliferation and invasion abilities decreased. Conclusion The expression of miRNA-375 in TNBC tissue and MDA-MB-231 is down-regulated. And miRNA-375 can inhibit the carcinogenesis and development of TNBC to a certain extent.

Key words: triple negative breast cancer, microRNA-375, invasiveness