上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

靶向敲除JARID2基因对葡萄膜黑色素瘤细胞生长与转移的影响

刘博1,2，邱纯2*，李鹏2，毕建军2，吴晴2，钮蓓蓓1   

  1. 上海交通大学 医学院 1.附属新华医院科研中心， 上海 200092； 2.附属第九人民医院眼科实验室， 上海 200011
  • 出版日期:2015-05-28 发布日期:2015-06-04
  • 通讯作者: 钮蓓蓓, 电子信箱: buby_niu@hotmail.com。
  • 作者简介:刘博（1985—）, 男， 硕士生; 电子信箱: cgxbead@126.com。 邱纯（1987—）， 男， 硕士生; 电子信箱: qiuchun2013@163.com。*共同第一作者。
  • 基金资助:

    国家自然科学基金（81372469， 81172323）

Effects of targeted knockout of JARID2 gene on growth and migration of uveal melanoma cells

LIU Bo1,2, QIU Chun2*, LI Peng2, BI Jian-jun2, WU Qing2, NIU Bei-bei1   

  1. 1.Scientific Research Center, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine，Shanghai  200092, China; 2.Laboratory of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
  • Online:2015-05-28 Published:2015-06-04
  • Supported by:

    National Natural Science Foundation of China， 81372469， 81172323

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摘要：

目的 探讨JARID2基因在葡萄膜黑色素瘤细胞生长和转移中的作用。方法 采用real-time PCR检测葡萄膜黑色素瘤细胞系OM431和SP6.5中JARID2 mRNA的表达。利用shRNA敲除OM431和SP6.5的JARID2基因，构建稳定转染细胞系shSP6.5和shOM431，Western blotting检测JARID2蛋白表达变化，观察靶向敲除JARID2基因对葡萄膜黑色素瘤细胞生长、转移及体外克隆形成的影响。结果 OM431和SP6.5细胞中JARID2 mRNA呈过表达。Western blotting检测结果显示：与OM431和SP6.5细胞比较，shOM431和shSP6.5细胞中JARID2蛋白表达显著减少。靶向敲除JARID2基因的葡萄膜黑色素瘤细胞的生长受到抑制，细胞转移数量减少，体外克隆形成率降低。结论 JARID2基因在葡萄膜黑色素瘤细胞的生长、转移和克隆形成能力中起着重要的作用。

关键词: JARID2基因, 葡萄膜黑色素瘤, 肿瘤转移

Abstract:

Objective To investigate the role of JARID2 gene in the growth and migration of uveal melanoma cells. Methods The mRNA expression of JARID2 in uveal melanoma cell lines OM431 and SP6.5 was detected by the real-time PCR. The JARID2 gene of OM431 and SP6.5 was knocked out by shRNA and stable transfected cell lines shSP6.5 and shOM431 were constructed. Variations of protein expression of JARID2 were detected by the Western blotting. Effects of targeted knockout of JARID2 gene on the growth, migration, and in vitro colony formation of uveal melanoma cells were observed. Results The mRNA expression of JARID2 in OM431 and SP6.5 was over-expressed. The results of Western blotting showed that compared with OM431 and SP6.5, the protein expression of JARID2 in shOM431 and shSP6.5 significantly decreased. Targeted knockout of JARID2 gene could inhibit the growth, migration, and in vitro colony formation of uveal melanoma cells. Conclusion JARID2 gene plays an important role in the growth, migration, and colony formation of uveal melanoma cells.

Key words: JARID2 gene, uveal melanoma, tumor metastasis