上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

新型干燥血栓微粒局灶性脑梗死模型的研究

陈凤，龙宗泓，李洪   

  1. 第三军医大学附属新桥医院麻醉科， 重庆 400037
  • 出版日期:2016-04-28 发布日期:2016-05-26
  • 通讯作者: 李洪, 电子信箱: Lh78553@126.com。
  • 作者简介:陈凤（1988—）， 女， 住院医师, 硕士; 电子信箱: 867484473@qq.com。
  • 基金资助:

    国家自然科学基金（81571870)

Study on a focal cerebral infarction model built by novel dry blood clots

CHEN Feng, LONG Zong-hong, LI Hong   

  1. Department of Anesthesiology, Xinqiao Hospital Affiliated to Third Military Medical University, Chongqing 400037, China
  • Online:2016-04-28 Published:2016-05-26
  • Supported by:

    National Natural Science Foundation of China， 81571870

PDF (PC)

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摘要：

目的 采用新型干燥血栓微粒建立兔局灶性脑梗死模型，探讨不同栓塞剂量与梗死部位和梗死程度的关系。方法 兔自体血通过自凝、烘干、碾磨和滤网过滤制得干燥血栓微粒。栓塞组兔经右侧颈内动脉注入不同剂量（2.5、5.0、7.5、10.0、12.5、15.0、17.5 mg组）的干燥血栓微粒，对照组兔则注入等量生理盐水，分别在栓塞后2、72 h行神经功能评级，栓塞前及栓塞后72 h检测血浆脑损伤因子S100B含量，栓塞后72 h处死动物取脑组织进行病理学检查。结果 干燥血栓微粒大小均一（直径100～150 μm），生理盐水稀释后分布均匀、不易成团、不易自溶，栓塞成功率达90.5%。栓塞后72 h，栓塞组血浆S100B水平显著高于栓塞前和对照组，脑组织水含量高于对照组；各栓塞剂量组实验兔的病死率、神经功能评级及脑梗死体积比与栓塞剂量呈正相关；TTC染色脑片显示栓塞2.5、5.0、7.5 mg组80%动物梗死灶位于右侧大脑皮质，而栓塞10.0、12.5、15.0 mg组动物双侧大脑皮质均出现梗死灶。结论 利用干燥血栓微粒成功建立兔脑梗死模型，方法简单、成功率高，2.5、5.0、7.5 mg和10.0、12.5、15.0 mg可分别模拟单、双侧脑梗死，为脑梗死的治疗研究提供了不同类型的临床前模型。

关键词: 脑梗死, 干燥血栓微粒, 兔

Abstract:

Objective To build a rabbit focal cerebral infarction model by using novel dry blood clots and discuss the correlation of different clot weights with infarction location and degree. Methods Rabbit blood was drawn and made into dry blood clots after coagulation, drying, grinding, and filtering. Rabbits in infarction groups received different clot weights (2.5, 5.0, 7.5, 10.0, 12.5, 15.0, and 17.5 mg) through right internal carotid artery (ICA), while rabbits in the control group received equivalent amount of normal saline. Neurological function was assessed 2 and 72 h after infarction. The level of brain injury factor S100B in plasma was measured before and 72 h after infarction. Rabbits were sacrificed 72 h after infarction and brain tissues were harvested for the pathological examination. Results Dry blood clots with the uniform size (100-150 μm in diameter) were distributed evenly and hard to conglobate and dissolve after normal saline dilution. The infarction rate was 90.5%. The plasma S100B level in infarction groups 72 h after infarction was significantly increased as compared with that before infarction and in the control group. The brain water content in infarction groups was higher than that in the control group. The mortality, neurological function score, and cerebral infarction volume ratio in infarction groups were positively correlated to clot weight. TTC staining brain slices showed that infarction foci in about 80% animals of infarction groups (2.5, 5.0, and 7.5 mg) located in right cerebral cortex, while infarction foci occurred in bilateral cerebral cortexes in animals of infarction groups (10.0, 12.5, and 15.0 mg). Conclusion The rabbit cerebral infarction model has been successfully built by using dry blood clots. The method is simple with high success rate. Unilateral or bilateral cerebral infarction can be simulated by administration of 2.5, 5.0, 7.5 mg or 10.0, 12.5, 15.0 mg blood clots. This study provides a different pre-clinical model for the investigation and treatment of cerebral infarction.

Key words: cerebral infarction, dry blood clots, rabbit