上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（临床研究） • 上一篇    下一篇

慢性丙型肝炎病毒感染者外周血滤泡辅助T淋巴细胞的变化研究

殷文伟1，童师雯2   

  1. 1.教育部感染性疾病分子生物学重点实验室，重庆医科大学病毒性肝炎研究所,重庆医科大学附属第二医院感染科， 重庆 400010； 2.重庆医科大学附属第二医院临床营养科，重庆 400010
  • 出版日期:2016-06-28 发布日期:2016-07-25
  • 通讯作者: 童师雯, 电子信箱: tsw129qq2001@163.com。
  • 作者简介: 殷文伟（1983—）， 男， 助理研究员, 博士; 电子信箱: ustcyww@163.com。
  • 基金资助:

    国家自然科学基金（81401664, 81301423)

Study on the changes in CD4+CXCR5+ follicular helper T lymphocytes in peripheral blood of patients with chronic hepatitis C

YIN Wen-wei1, TONG Shi-wen2   

  1. 1.Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010， China； 2.Department of Clinical Nutrition, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010， China
  • Online:2016-06-28 Published:2016-07-25
  • Supported by:

    National Natural Science Foundation of China, 81401664, 81301423

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摘要：

目的 研究滤泡辅助T淋巴细胞（TFH细胞）在慢性丙型肝炎病毒（HCV）感染者外周血的变化特点及其与疾病特征的相关性。方法 共纳入20名健康对照者和23例HCV感染者，其中4例接受聚乙二醇干扰素α联合利巴韦林治疗。流式细胞术检测外周血CD4+CXCR5+ TFH细胞比例变化。结果 慢性HCV感染者外周血CD4+CXCR5+ TFH细胞占单个核细胞的比例为5.845%（2.680%~14.200%），健康对照组为3.765%（1.200%~8.520%）（u=124.5, P=0.010 6）；占CD4+T淋巴细胞的比例，感染组和对照组分别为14.800%（8.440%~36.600%）和9.550%（2.700%~21.800%）（u=132.0,P=0.017 6）。CD4+CXCR5+ TFH细胞比例与HCV RNA载量呈负相关（r=-0.432 8,P=0.039 1)，与GPT和GOT无相关性。抗病毒治疗后HCV感染者TFH细胞比例降低。结论 CD4+CXCR5+ TFH细胞可能参与了慢性HCV感染机体抗病毒免疫应答。

关键词: 丙型肝炎病毒, 滤泡辅助T细胞, 病毒载量, 谷丙转氨酶

Abstract:

Objective To investigate changes in characteristics of CD4+CXCR5+ follicular helper T lymphocytes (TFH cells) in peripheral blood of patients with chronic hepatitis C virus (HCV) infection and its correlation with the disease characteristics. Methods Twenty-three patients with HCV infection and 20 healthy controls were enrolled and 4 patients received a pegylated interferon-α and ribavirin combination treatment. The changes in the proportion of peripheral blood CD4+CXCR5+ TFH cells were assessed by flow cytometry. Results The percentage of CD4+CXCR5+ TFH cells in peripheral blood mononuclear cells was 5.845% (2.680%-14.200%) for patients with chronic HCV infection and 3.765% (1.200%-8.520%) for healthy controls (u=124.5, P=0.010 6). The proportion of TFH cells in CD4+T cells was 14.800% (8.440%-36.600%) for patients and 9.550% (2.700%-21.800%) for healthy controls (u=132.0, P=0.017 6). The proportion of CD4+CXCR5+ TFH cells were negatively correlated to HCV RNA load (r=-0.432 8, P=0.039 1) and showed no correlation with serum glutamic pyruvic transaminase or glutamic oxaloacetic transaminase levels. The anti-HCV therapy decreased the proportion of CD4+CXCR5+ TFH cells in HCV-infected patients. Conclusion CD4+CXCR5+TFH cells may participate in the anti-viral immune response in the process of chronical infection with HCV.

Key words: hepatitis C virus, follicular helper T lymphocytes, HCV RNA, alanine aminotransferase