上海交通大学学报（医学版）

上海交通大学学报（医学版）

• 论著（基础研究） • 上一篇    下一篇

大肠埃希菌蛋白质组芯片对砷相互作用蛋白的检测分析

刘殷 1，杨丽娜 2，张海南 1，陶生策 1   

  1. 1. 上海交通大学系统生物医学研究院，系统生物医学教育部重点实验室，上海 200240；2. 复旦大学附属肿瘤医院中西医结合科，上海 200032
  • 出版日期:2017-05-28 发布日期:2017-05-31
  • 通讯作者: 陶生策，电子信箱：taosc@sjtu.edu.cn。
  • 作者简介:刘殷（1992—），女，硕士生；电子信箱：yinliu_lynn@outlook.com。
  • 基金资助:

    国家自然科学基金（31670831）

Identification and analysis of arsenic interactors by Escherichia coli proteome microarray

LIU Yin1, YANG Li-na2, ZHANG Hai-nan1, TAO Sheng-ce1   

  1. 1. Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China; 2. Department of Integrative Oncology, Fudan University Shanghai Cancer Center, Shanghai 200032, China
  • Online:2017-05-28 Published:2017-05-31
  • Supported by:

    National Natural Science Foundation of China, 31670831

PDF (PC)

872

摘要：

目的 ·以大肠埃希菌作为模式生物，全局性分析砷对大肠埃希菌的作用及生理途径的影响。方法 ·基于由4 256个大肠埃希菌蛋白构建的蛋白质组芯片，检测并验证三价砷化物与大肠埃希菌蛋白之间的相互作用。通过生物信息学对砷的相互作用蛋白的功能及相互作用网络进行分析。结果 ·发现了91个与砷有显著相互作用的蛋白。经过生物信息学分析发现这些蛋白涉及多种具有催化活性的酶类，并且显著影响着大肠埃希菌的细胞合成及代谢途径。通过Western blotting验证了 malY、cfa及hypF基因编码的蛋白与砷之间的结合，进一步证实了蛋白质芯片的结果。结论 ·砷与大肠埃希菌的多种酶类有相互作用，并对其生理代谢途径有显
著影响。

关键词:  , 砷；蛋白质组芯片；大肠埃希菌；生理代谢

Abstract:

Objective · To globally study the influence of arsenite to the various biological pathways of Escherichia coli as a model organism. Methods · The protein-arsenite interactions was globally studied based on a proteome microarray constructed by 4 256 affinity-purified Escherichia coli proteins. The functions of interacting proteins and their network were then analyzed by bioinformatics. Results · 91 proteins that remarkably interact with arsenic were successfully identified. Bioinformatics analysis found that most of the proteins possess catalytic activity and are involved in various biosynthesis and cellular metabolism pathways. The interactions of arsenic with proteins encoded by malY, cfa and hypF genes were further validated by Western blotting, which proves the results of proteome microarray reliable. Conclusion · Arsenite interacts with a variety of enzymes of Escherichia coli and can greatly affect its biological metabolism.

Key words: arsenic, proteome microarray, Escherichia coli, biological metabolism