上海交通大学学报(医学版) ›› 2017, Vol. 37 ›› Issue (6): 732-.doi: 10.3969/j.issn.1674-8115.2017.06.004

• 论著(基础研究) • 上一篇    下一篇

维生素 E 拮抗双酚 A 对卵巢颗粒细胞性激素分泌的抑制作用

陈威威,孙伟杰,王磊,焦先婷,余晓丹   

  1. 上海交通大学 医学院附属新华医院环境与儿童健康教育部和上海市重点实验室,上海 200092
  • 出版日期:2017-06-28 发布日期:2017-07-05
  • 通讯作者: ?余晓丹,电子信箱:xd_yu2003@126.com
  • 作者简介:陈威威(1990—),男,硕士生;电子信箱:chenweiwei1990@yahoo.com
  • 基金资助:

    国家重点基础研究发展计划(“973”计划)(2014CB943300);上海市教育委员会高峰高原学科建设计划(20152220)

Vitamin E could reverse effects of bisphenol A on steroidogenesis in rat ovary granulosa cells

CHEN Wei-wei, SUN Wei-jie, WANG Lei, JIAO Xian-ting, YU Xiao-dan   

  1. MOE-Shanghai Key Lab of Children’s Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
  • Online:2017-06-28 Published:2017-07-05
  • Supported by:

    National Key Basic Research Program of China, “973” Program,  2014CB943300; Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Grant Support, 20152220

摘要:

目的 · 研究维生素E 能否对双酚A(BPA)的雌性性激素干扰效应起拮抗作用,探索拮抗作用的适宜浓度。方法 · 提取大鼠 卵巢原代颗粒细胞。不同浓度 BPA(0、0.01、0.1、1、10、100 μmol/L)孵育 48 h 后,收集并用 ELISA 法测定细胞培养基中的雌二醇 (E2)及孕酮(P4)浓度。采用健康女性卵泡液中的平均浓度(简称“人体平均浓度”,5 μmol/L)或高浓度(25 μmol/L)维生素E(α生育酚 ) 单独孵育或与 BPA(10、100 μmol/L)共同孵育颗粒细胞48 h 后,测定培养液中性激素浓度。结果 · 10 μmol/L 及 100 μmol/L BPA 可使大鼠原代卵巢颗粒细胞分泌性激素水平呈浓度依赖性降低(P<0.05)。 25 μmol/L 维生素 E 单独孵育时可分别使 E2 和 P4 浓度 显著升高(44.89±15.18)% 和(43.33±8.82)%(P<0.05);维生素 E(5 μmol/L 或 25 μmol/L)和 BPA 共同孵育时,性激素浓度恢复 正常,与阴性对照组相比,差异无统计学意义(P>0.05)。 结论 · 人体平均浓度及高浓度维生素 E 均可拮抗 BPA 导致的性激素降低效 应,而高浓度维生素 E 的改善效果相对显著。

关键词: 双酚 A, 维生素 E, 卵巢颗粒细胞, 雌二醇, 孕酮

Abstract:

 Objective · To investigate whether vitamin E could reverse the disruptive effects of bisphenol A (BPA) on steroidogenesis and to explore the optimal vitamin E concentration.  Methods · Rat primary granulosa cells were extracted and exposed to BPA (0, 0.01, 0.1,1,10,100 μmol/L). After 48 h of incubation, culture medium was collected and estradiol (E2) and progesterone (P4) were measured using ELISA kits. Then, granulosa cells were incubated with 5 μmol/L(average concentration in follicular fluid) or 25 μmol/L (high concentration in follicular fluid) vitamin E (α-tocopherol) or vitamin E (5 μmol/ L or 25 μmol/L) plus BPA (10 μmol/L or 100 μmol/L) for 48 h, E2 and P4 were measured.  Results · BPA at 10 μmol/L and 100 μmol/L suppressed E2 and P4 production in a dose-dependent manner (P<0.05). Vitamin E at 25 μmol/L significantly increased E2 and P4 levels by (44.89±15.18) % and (43.33±8.82) % (P<0.05), respectively. Coincubation of the granulosa cells with BPA and vitamin E (5 μmol/L or 25 μmol/L) restored the productions of E2 and P4, which were not significantly different from the control group (P>0.05).  Conclusion · Vitamin E (5 μmol/L/ 25 μmol/L) could reverse BPA-induced reduction in steroid hormone production in rat ovary granulosa cells, and the antagonistic effect of 25 μmol/L vitamin E was more obvious than that of 5 μmol/L vitamin E.

Key words: bisphenol A , vitamin E, ovary granulosa cell, estradiol, progesterone