上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2017, Vol. 37 ›› Issue (12): 1616-.doi: 10.3969/j.issn.1674-8115.2017.12.006

• 论著（基础研究） • 上一篇    下一篇

胰岛素样因子6对单侧输尿管梗阻小鼠肾脏纤维化和钙化的影响

高磊，吴世勇，蒋丹，王颖，李勇湧，王德伟，常广磊，张鹏，覃铮，张冬颖   

  1. 重庆医科大学附属第一医院  1. 心血管内科，2. 实验研究中心，重庆  400016
  • 出版日期:2017-12-28 发布日期:2018-01-10
  • 通讯作者: 张冬颖，电子信箱：zdy.chris@qq.com
  • 作者简介:高磊（1991—），男，硕士生；电子信箱：653161583@qq.com
  • 基金资助:
    国家自然科学基金（81570212）；重庆市卫生和计划生育委员会中医药科技项目（ZY201702073）；重庆市研究生科研创新项目（CYB15093）；心脏康 复与代谢治疗研究基金；中国心血管健康联盟心血管健康研究院“进阶”研究基金项目

Effects of insulin-like peptide 6 on renal fibrosis and calcification in unilateral ureteral obstruction mice#br#

GAO Lei, WU Shi-yong, JIANG Dan, WANG Ying, LI Yong-yong, WANG De-wei, CHANG Guang-lei, ZHANG Peng, QIN Zheng, ZHANG Dong-ying   

  1. 1. Department of Cardiovascular Medicine, 2. Department of Laboratory Research Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
  • Online:2017-12-28 Published:2018-01-10
  • Supported by:
    National Natural Science Foundation of China, 81570212; Chinese Medicine Science and Technology Project of Chongqing Health and Family Planning Commission, ZY201702073; Postgraduate Research and Innovation Project of Chongqing, CYB15093; Cardiac Rehabilitation and Metabolic Therapy Research Foundation; "Advanced" Research Foundation Project of Cardiovascular Health Institute, China Cardiovascular Health Alliance

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摘要： 目的 · 探讨胰岛素样因子6（Insl6）对单侧输尿管梗阻（UUO）小鼠肾脏纤维化和钙化的影响。方法 · SPF 级 C57BL/6 雄 性小鼠24 只，随机分为3 组，假手术组（n=8）、 UUO+ 生理盐水组（n=8）和UUO+Insl6 组（n=8）。 UUO 模型建立后第10 日处死 小鼠，取术侧肾组织。采用天狼星红染色、Masson 染色、茜素红 S 染色分别检测肾脏胶原沉积及钙化程度，Western blotting 检测肾 脏 TGF-β1 表达，realtime-PCR 检测肾脏TGF-β1、BMP2、Col1a1、Col2a1 mRNA 表达。结果 · 与假手术组相比，UUO+ 生理盐水 组肾脏胶原面积百分比显著增加，以Ⅰ型胶原为主，钙盐沉积，TGF-β1 蛋白表达，TGF-β1、BMP2、Col1a1、Col2a1 mRNA 表达均 显著增加（均P<0.05）。与 UUO+ 生理盐水组相比，UUO+Insl6 组小鼠肾脏胶原面积，钙盐沉积，TGF-β1 蛋白，TGF-β1、BMP2、 Col1a1、Col2a1 mRNA 表达均显著降低（均P<0.05）。 结论 · Insl6 具有抗UUO 诱导的肾脏纤维化及钙化作用，其机制可能与调控 TGF-β1、collagen Ⅰ、BMP2、collagen Ⅱ表达水平有关。

关键词: 肾脏纤维化, 肾脏钙化, 单侧输尿管梗阻, 胰岛素样因子 6

Abstract:

Objective · To investigate the effects of insulin-like peptide 6 (Insl6) on renal fibrosis and calcification in unilateral ureteral obstruction (UUO) mice.  Methods · Twenty-four SPF male mice with genotypic background of C57BL/6 were divided into Sham (n=8), UUO+saline (n=8) and UUO+Insl6 (n=8) groups randomly. Mice were sacrificed 10 days after operation and renal tissues of surgical side were obtained. Sirus red staining, Masson staining and alizarin red S staining were used to verify the level of collagen and calcium deposition. TGF-β1 expression was determined by Western blotting. Realtime-PCR was used for determining TGF-β1, BMP2, Col1a1, and Col2a1 mRNA expression.  Results · Compared with sham group, fibrotic area especially collagen Ⅰ, calcium deposition, TGF-β1 protein, and TGF-β1, BMP2, Col1a1, and Col2a1 mRNA expression in UUO+saline group significantly
 increased (all P<0.05). As compared with UUO+saline group, fibrotic area especially collagen Ⅰ, calcium deposition, TGF-β1 protein, and TGF-β1,
 BMP2, Col1a1, and Col2a1 mRNA expression in UUO+Insl6 group significantly decreased (all P<0.05).  Conclusion · Insl6 inhibits UUO-induced renal fibrosis and calcification, which may be related to regulation of TGF-β1, collagen Ⅰ , BMP2 and collagen Ⅱ expression levels.

Key words: renal fibrosis, renal calcification, unilateral ureteral obstruction, insulin-like peptide 6