上海交通大学学报（医学版）

上海交通大学学报（医学版） ›› 2017, Vol. 37 ›› Issue (12): 1623-.doi: 10.3969/j.issn.1674-8115.2017.12.007

• 论著（基础研究） • 上一篇    下一篇

黄酮类化合物影响大麻素受体过表达细胞中 cAMP 含量的初步研究

李婷 1, 2，刘坚华 1   

  1. 1. 上海交通大学  基础医学院化学教研室，上海 200025；2. 上海市浦东新区周浦医院药剂科，上海 201318
  • 出版日期:2017-12-28 发布日期:2018-01-10
  • 通讯作者: ?刘坚华，电子信箱：jhliu7912@shsmu.edu.cn
  • 作者简介:李婷（1979—），女，主管药师，学士；电子信箱：zhoupuliting@163.com
  • 基金资助:
    国家自然科学青年基金（81402775）；高等学校博士学科点专项科研基金（20130073120113）

Preliminary study of flavonoids affecting cAMP level in cannabinoid receptor overexpressed cells#br#

LI Ting1, 2, LIU Jian-hua1   

  1. 1. Department of Chemistry, Basic Medicine Faculty of Shanghai Jiao Tong University, Shanghai 200025, China; 2. Department of Pharmacy, Shanghai Pudong New District Zhoupu Hospital, Shanghai 201318, China
  • Online:2017-12-28 Published:2018-01-10
  • Supported by:
    National Natural Science Youth Fund of China, 81402775； Special Research Fund for Doctoral Programs in Colleges and Universities, 20130073120113

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摘要： 目的 · 初步探讨黄酮类化合物对大麻素受体（CBR）过表达细胞中环磷酸腺苷（cAMP）含量的影响。方法 · 构建重组表达质 粒 GV144-CB1R 和 GV144-CB2R；转染 HEK-293 细胞建立 CBR 过表达细胞模型，研究黄酮类化合物对 CBR 过表达细胞中 cAMP 含 量的影响。结果 · 成功构建 CBR 过表达细胞模型。化合物作用结果显示，所测试的化合物在测试浓度下均可使过表达CBR 的 HEK293 细胞中 cAMP 含量显著降低，且对 CB2R 过表达的细胞有一定选择性。结论 · 该研究所使用的黄酮类化合物都可以在 μmol/L 浓度 下降低 CBR 过表达细胞中 cAMP 的含量。

关键词: 大麻素受体, 黄酮类化合物, 大麻素受体过表达细胞, 环磷酸腺苷

Abstract:

Objective · To evaluate the effects of flavonoids on cyclic adenosine monophosphate (cAMP) level in cannabinoid receptor (CBR) overexpressed cells.  Methods · Eukaryotic expression vectors GV144-CB1R and GV144-CB2R were constructed, and transfected into HEK-293 cells to establish CBR overexpressed cell lines. Several flavonoid compounds were studied by measuring the change of cAMP level in CBR-overpressed HEK293 cell lines.  Results · The CBR overexpressed cell lines were constructed successfully. cAMP level in CBR-overexpressed HEK-293 cells decreased after incubation with flavonoid compounds. And these effects were selective for CB2R overexpressed cells.  Conclusion · All flavonoids studied exhibited decreasing effect on cAMP level in CBR-overexpressed cells at μmol/L level.

Key words: cannabinoid receptor, flavonoids/flavonoid compounds, cannabinoid receptor overexpressed cell, cyclic adenosine monophosphate