›› 2009, Vol. 29 ›› Issue (7): 767-.

• 专题报道 (儿童保健学) • 上一篇    下一篇

生命早期前爪感觉和精细动作剥夺对大鼠空间学习记忆的影响

张媛媛1, 李  斐2, 曹晓华3, 金星明2, 颜崇淮1, 田 英1,4, 沈晓明1   

  1. 1. 上海交通大学 医学院新华医院 上海市儿科医学研究所 上海市环境与儿童健康重点实验室, 上海 200092; 2. 上海交通大学 医学院上海儿童医学中心发育行为儿科 上海市环境与儿童健康重点实验室, 上海 200127; 3. 华东师范大学 上海市脑功能基因组学重点实验室, 上海 200062; 4. 上海交通大学 公共卫生学院环境与劳动卫生教研室, 上海 200025
  • 收稿日期:2009-02-16 出版日期:2009-07-25 发布日期:2009-07-25
  • 通讯作者: 沈晓明, 电子信箱: xmshen@shsmu.edu.cn
  • 作者简介:张媛媛(1983—), 女, 博士生;电子信箱: zhy-yuan@163.com。
  • 基金资助:

    国家自然科学基金(30672272);上海市科委重大基础研究项目(05DJ14007)

Effects of forepaw sensorimotor deprivation in early life on spatial learning and memory in rats

ZHANG Yuan-yuan1, LI Fei2, CAO Xiao-hua3, JIN Xing-ming2, YAN Chong-huai1,  TIAN Ying1,4, SHEN Xiao-ming1   

  1. 1.Shanghai Institute for Pediatric Research, Shanghai Key Laboratory of Children's Environmental Health, Xinhua Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China;2. Department of Developmental and Behavioral Pediatrics, Shanghai Key Laboratory of Children's Environmental Health, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, China;3. Shanghai Institute of Brain Functional Genomics, East China Normal University, Shanghai 200062, China;4. Department of Environmental Health, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China
  • Received:2009-02-16 Online:2009-07-25 Published:2009-07-25
  • Supported by:

    National Natural Science Foundation of China, 30672272; Shanghai Science and Technology Committee Foundation, 05DJ14007

摘要:

目的 研究生命早期前爪感觉和精细动作剥夺对大鼠海马依赖性空间参考学习记忆能力的影响。方法 新生SD大鼠随机分为前爪感觉和精细动作剥夺组(实验组,n=53)和假手术组(对照组,n=55)。取13日龄(PN13)实验组大鼠,运用显微外科技术建立幼鼠前爪感觉和精细动作剥夺模型。于PN25(PN21~31)、PN35(PN31~39)、PN45(PN41~50)和PN60(PN56~64)4个时间段,对两组大鼠分别进行旷场测试评估自发活动和探索能力;Morris 水迷宫测试评估空间参考学习记忆能力。结果 旷场测试中,两组大鼠于各时间段的自发活动各项指标比较差异无统计学意义 (P>0.05)。Morris水迷宫测试显示,在PN25和PN35时间段,对照组大鼠训练和测试阶段的成绩均显著高于实验组 (P<0.05);在PN45时间段,训练阶段两组大鼠成绩比较差异无统计学意义(P>0.05),测试阶段对照组大鼠成绩显著高于实验组 (P<0.05)。结论 生命早期前爪感觉和精细动作剥夺,对大鼠自发性粗大运动能力无明显影响,但可损伤其空间参考学习记忆能力。

关键词: 感觉和精细动作剥夺, 海马, Morris水迷宫, 学习记忆

Abstract:

Objective To explore the effects of forepaw sensorimotor deprivation in early life on hippocampus-dependent spatial reference learning and memory in rats. Methods Newborn SD rats were randomly assigned to experiment group (deprivation of forepaw sensorimotor function, n=53) and control group(n=55). Rats of postnatal day 13 (PN13) in experiment group were selected, and models of forepaw sensorimotor deprivation were established by microsurgical technique. Open field tests and Morris water maze tests were performed during the time periods of PN25(PN21-31),PN35(PN31-39),PN45(PN41-50) and PN60(PN56-64) to evaluate the locomotor activity and spatial reference learning and memory, respectively. Results In open field tests, there was no significant difference in parameters of locomotor activity and exploratory behavior between the two groups (P>0.05). In Morris water maze tests, control group performed significantly better than experiment group during training sessions and probe tests on PN25 and PN35 (P<0.05). While on PN45, although there was no significant difference between the two groups during training sessions, control group performed significantly better than experiment group during probe tests (P<0.05). Conclusion The deprivation of forepaw sensorimotor in early life has no significant effect on the locomotor activity and exploratory behavior of rats, but can impair the spatial reference learning and memory.

Key words: sensorimotor deprivation, hippocampus, Morris water maze, learning and memory

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