上海交通大学学报（医学版）

›› 2009, Vol. 29 ›› Issue (12): 1459-.

• 论著（基础研究） • 上一篇    下一篇

不同剂量托吡酯对癫癎大鼠海马组织中NCAM和GAP-43 mRNA表达的影响

吴丹红1, 钟 萍2, 包关水1, 周 红1, 沈 珺1, 黄菲菲1   

  1. 1. 上海交通大学 医学院第三人民医院神经内科, 上海 201900；2. 上海市中西医结合医院神经内科, 上海 200083
  • 出版日期:2009-12-25 发布日期:2009-12-25
  • 作者简介:吴丹红（1971—）, 女, 副主任医师, 硕士；电子信箱: kathywuxue@sina.com。
  • 基金资助:

    上海高校选拔培养优秀青年教师科研专项基金(07JWYQ02）

Effects of different doses of topiramate on expression of NCAM and GAP-43 mRNA in hippocampus of rats with epilepsy

WU Dan-hong1, ZHONG Ping2, BAO Guan-shui1, ZHOU Hong1, SHEN Jun1, HUANG Fei-fei1   

  1. 1. Department of Neurology, The Third People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 201900, China；2. Department of Neurology, Shanghai Traditional Chinese Medical Integrated Hospital, Shanghai 200083, China
  • Online:2009-12-25 Published:2009-12-25
  • Supported by:

    Foundation for Excellent Young Higher Education Teachers of Shanghai, 07JWYQ02

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摘要：

目的 研究不同剂量托吡酯（TPM）干预下，大鼠海马组织中神经细胞黏附分子（NCAM）和生长相关蛋白-43（GAP-43）mRNA的表达变化。方法 将48只大鼠随机分成正常对照组、海人酸（KA）组、10 mg/kg TPM组、40 mg/kg TPM组、100 mg/kg TPM组和400 mg/kg TPM组，每组8只；建立不同剂量的TPM干预癫癎大鼠模型。观察大鼠行为学改变；通过Real-time PCR测定各组大鼠海马组织中NCAM和GAP-43的mRNA表达。结果 KA组大鼠海马NCAM及GAP-43的mRNA表达水平明显高于正常对照组（P<0.01），10 mg/kg TPM组和40 mg/kg TPM组与KA组的差异无统计学意义，100 mg/kg TPM组和400 mg/kg TPM组明显低于KA组（P<0.01），其中400 mg/kg TPM组明显低于100 mg/kg TPM组（P<0.01）。结论 KA诱导癫癎大鼠海马NCAM和GAP-43 mRNA表达上调，较大剂量TPM能够抑制NCAM和GAP-43 mRNA的表达，且抑制作用与TPM剂量有关。

关键词: 癫癎, 托吡酯, 神经细胞黏附分子, 生长相关蛋白-43

Abstract:

Objective To explore the effects of different doses of topiramate (TPM) on the expression of nerve cell adhesion molecule (NCAM) and growth-associated protein 43 (GAP-43) mRNA in hippocampus of rats with epilepsy. Methods Forty-eight rats were randomly divided into normal control group, kainic acid (KA) group, 10 mg/kg TPM group, 40 mg/kg TPM group, 100 mg/kg TPM group and 400 mg/kg TPM group (n=8). The models of rats with epilepsy treated by different doses of TPM were established. The behavior of rats was observed, and the expression of NCAM and GAP-43 mRNA in hippocampus of rats was determined by Real-time PCR. Results The expression of NCAM and GAP-43 mRNA in KA group was significantly higher than that in normal control group (P<0.01), while there was no significant difference between 10 and 40 mg/kg TPM groups and KA group, that in 100 and 400 mg/kg TPM groups was significantly lower than that in KA group (P<0.01), and that in 400 mg/kg TPM group was significantly lower than that in 100 mg/kg TPM group (P<0.01). Conclusion KA can up-regulate the expression of NCAM and GAP-43 mRNA in hippocampus of rats with epilepsy. Higher dose of TPM can inhibit the expression of NCAM and GAP-43 mRNA, and the inhibitory effect is related with the dose of TPM.

Key words: epilepsy, topiramate, nerve cell adhesion molecule, growth-associated protein 43