上海交通大学学报（医学版）

›› 2010, Vol. 30 ›› Issue (6): 703-.

• 论著（临床研究） • 上一篇    下一篇

系统性红斑狼疮患者外周血单个核细胞单链DNA表达研究

赵宝景, 黄新芳, 沈 南, 陈顺乐, 王 元   

  1. 上海交通大学 医学院仁济医院风湿免疫科, 上海 200001
  • 出版日期:2010-06-25 发布日期:2010-06-28
  • 通讯作者: 王 元, 电子信箱: wangyuan1950@yahoo.com.cn。
  • 作者简介:赵宝景（1982—）, 男, 硕士生；电子信箱: zhao_bao_jing@yahoo.cn。
  • 基金资助:

    国家自然科学基金（30301026）；上海市重点学科建设项目资助 (T0203)；上海市优秀学科带头人计划（07XD14021）；上海市医院临床科研资源共享平台项目 (SHDC12007205)

Expression of single-stranded DNA in peripheral blood mononuclear cells in patients with systemic lupus erythematosus

ZHAO Bao-jing, HUANG Xin-fang, SHEN Nan, CHEN Shun-le, WANG Yuan   

  1. Department of Rheumatology,Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200001, China
  • Online:2010-06-25 Published:2010-06-28
  • Supported by:

    National Natural Science Foundation of China, 30301026；Shanghai Key Discipline Construction Project, T0203；Shanghai Excellent Academic Leader Plan, 07XD14021；Shanghai Clinical Research Resource Sharing Platform Project, SHDC12007205

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摘要：

目的 研究系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)中单链DNA（ssDNA）表达情况。方法 流式细胞术检测26例SLE患者（SLE组）外周血ssDNA表达阳性的PBMC细胞百分比（ssDNA阳性率），设立正常对照组（n=17）和其他结缔组织病（CTD）对照组（n=7）。结果 SLE组、正常对照组和CTD对照组外周血B淋巴细胞中ssDNA阳性率分别为(0.906±0.838)%、(0.216±0.371)%和(0.270±0.311)%。统计学分析表明，SLE组患者外周血B淋巴细胞中ssDNA阳性率显著高于两对照组（P<0.01, P<0.05）；SLE组内有与无狼疮肾炎患者的外周血B淋巴细胞中ssDNA阳性率分别为（1.228±0.259）%和（0.510±0.436）%，两者比较差异有统计学意义（P<0.05）。结论 SLE和狼疮肾炎的发生和发展可能与患者外周血表达ssDNA的B淋巴细胞异常增多有关。

关键词: 单链DNA, 外周血单个核细胞, 系统性红斑狼疮, 狼疮肾炎

Abstract:

Objective To investigate the expression of single-stranded DNA (ssDNA) in peripheral blood mononuclear cells (PBMC) in patients with systemic lupus erythematosus (SLE). Methods Flow cytometry was employed to detect the percentages of PBMC with positive ssDNA expression in 26 patients with SLE (SLE group), and normal control group (n=17) and the other connective tissue diseases (CTD) control group (n=7) were established. Results The ssDNA positive rates in peripheral B lymph cells in SLE group, normal control group and CTD control group were (0.906±0.838)%, (0.216±0.371)% and (0.270±0.311)%, respectively. The ssDNA positive rate in peripheral B lymph cells in SLE group was significantly higher than those in normal control group and CTD control group (P<0.01, P<0.05). There were significant differences in the ssDNA positive rates in peripheral B lymph cells between patients with lupus nephritis in SLE group and those without lupus nephritis in SLE group [(1.228±0.259)% vs (0.510±0.436)%, P<0.05]. Conclusion The development of SLE and lupus nephritis may be related to the increase of peripheral B lymph cells with ssDNA expression.

Key words: single-stranded DNA, peripheral blood mononuclear cell, systemic lupus erythematosus, lupus nephritis