上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (1): 15-.doi: 10.3969/j.issn.1674-8115.2011.01.004

• 论著（基础研究） • 上一篇    下一篇

荧光纳米颗粒NaYF4:Yb,Er作为显像介质的生物相容性

虞永江1, 马晓荣1, 于国鹏1, 高同斌1, 齐 隽1, 陈 方2   

  1. 1.上海交通大学 医学院附属新华医院泌尿外科, 上海 200092；2.上海交通大学附属儿童医院泌尿外科, 上海 200040
  • 出版日期:2011-01-28 发布日期:2011-02-01
  • 通讯作者: 陈 方, 电子信箱: chenfang007@hotmail.com。
  • 作者简介:虞永江（1977—）, 男, 主治医师, 博士生；电子信箱: dyongyong@126.com。
  • 基金资助:

    上海交通大学医学院附属新华医院基金（09QYJ03）

Biocompatibility of fluorescent nanoparticles NaYF4:Yb,Er as imaging media

YU Yong-jiang1, MA Xiao-rong1, YU Guo-peng1, GAO Tong-bin1, QI Juan1, CHEN Fang2   

  1. 1.Department of Urology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China；2.Department of Urology, Shanghai Children's Hospital, Shanghai Jiaotong University, Shanghai 200040, China
  • Online:2011-01-28 Published:2011-02-01
  • Supported by:

    Foundation from Xinhua Hospital, Shanghai Jiaotong University School of Medicine, 09QYJ03

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摘要：

目的 检测上转频荧光纳米颗粒的生物学体内、外相容性，证实其作为显像介质的生物安全性。方法 将培育后的小鼠骨髓间充质干细胞（BMSC）、胚胎成纤维细胞（NIH/3T3）及成肌细胞（C2C12）分别与不同浓度（0、10、50、100、200 μg/mL）的NaYF4:Yb,Er共孵育，采用MTT法检测细胞的增殖活性，并测定C2C12成肌细胞形成肌管细胞的功能。将NaYF4:Yb,Er纳米颗粒DMEM混悬液注射入C57BL/6小鼠，行小鼠肝肾功能测定；并对重要脏器行HE组织学染色，检测小鼠的体内毒性。结果 MTT法细胞毒性检测显示，NaYF4:Yb,Er纳米颗粒对NIH/3T3和C2C12的毒性呈剂量与孵育时间正相关性（NIH/3T3：r=0.974，P<0.05；C2C12：r=0.996，P<0.05）；而对BMSC的毒性并没有表现出剂量与孵育时间具有相关性（r=-0.218，P>0.05）。NaYF4:Yb,Er纳米颗粒孵育48 h后，对C2C12成肌细胞形成肌管细胞功能未见明显影响。小鼠被注射NaYF4:Yb,Er纳米颗粒DMEM混悬液2、4周后，肝肾功能及重要脏器均未见明显损伤。结论 NaYF4:Yb,Er纳米颗粒作为具有强荧光强度的显影介质，在体内、外显影需求浓度下对细胞毒性及全身各重要器官的损伤均在安全范围内，是一种优秀的生物成像显影介质。

关键词: 上转频荧光纳米颗粒, NaYF4:Yb,Er, 生物显像介质, 生物相容性

Abstract:

Objective To investigate the biocompatibility of upconversion fluorescent nanoparticles in vivo and in vitro, and verify its safety as imaging media. Methods Mouse bone mesenchymal stem cells (BMSC), mouse embryonic fibroblasts (NIH/3T3) and primary myoblasts (C2C12) were incubated with different concentrations of NaYF4:Yb,Er (0,10,50,100 and 200 μg/mL). Cell proliferation was determined by MTT assay, and the formation of myotube cells from C2C12 myoblasts was detected. DMEM with NaYF4:Yb,Er nanoparticles were injected into C57BL/6 mice, and liver function and renal function were examined. HE staining was performed for main body organs, and toxicity was detected. Results MTT assay revealed that the cytotoxicity of NaYF4:Yb,Er on NIH/3T3 and C2C12 was positively correlated with incubation dose and time （NIH/3T3: r=0. 974, P<0.05; C2C12: r=0. 996, P<0.05）, while the same result was not found for BMSC (r=-0.218, P>0.05).The formation of myotube cells from C2C12 myoblasts was not significantly affected by incubation with NaYF4:Yb,Er for 48 h. No obvious damage of liver and renal function and main body organs was observed after injection of DMEM with NaYF4:Yb,Er nanoparticles in mice. Conclusion As biological luminescent labels with strong intensity, NaYF4: Yb, Er has less toxicity both in vivo and in vitro to the requirement of imaging, and is an ideal biological imaging media.

Key words: upconversion fluorescent nanoparticles, NaYF4:Yb,Er, biological imaging media, biocompatibility