白陶土诱导新生大鼠梗阻性脑积水模型的建立

doi:10.3969/j.issn.1674-8115.2011.01.008

›› 2011, Vol. 31 ›› Issue (1): 35-.doi: 10.3969/j.issn.1674-8115.2011.01.008

白陶土诱导新生大鼠梗阻性脑积水模型的建立

吴少峰¹, 杨波¹, 金惠明², 鲍南¹

1.上海交通大学医学院附属儿童医学中心外科, 上海 200127；2.上海交通大学医学院附属新华医院儿外科, 上海 200092

出版日期:2011-01-28 发布日期:2011-02-01
通讯作者: 杨波, 电子信箱: yangbosh@gmail.com。
作者简介:吴少峰（1977—）, 男, 住院医师, 博士；电子信箱: wusf2411@gmail.com。

Establishment of newborn rat model of obstructive hydrocephalus by kaolin injection

WU Shao-feng¹, YANG Bo¹, JIN Hui-ming², BAO Nan¹

1.Department of Surgery, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China；2.Department of Pediatric Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China

Online:2011-01-28 Published:2011-02-01

摘要/Abstract

摘要：

目的采用白陶土混悬液诱导的方法建立新生大鼠梗阻性脑积水模型。方法对出生1 d的SD大鼠经枕大池注射白陶土混悬液（实验组，n=40），另设生理盐水对照组（n=10）和正常对照组（n=10）；再将实验组分为4个亚组（n=10），分别在注射后第1、2、3、4周进行观察。注射后第1、2、3、4周，对大鼠进行头颅磁共振成像（MRI）检查。1月龄时，对大鼠开始Morris水迷宫训练，观察大鼠空间辨别能力和记忆能力。结果实验组大鼠逐渐出现弓背、宽基步态等共济失调的表现。注射白陶土1周时，实验组大鼠MRI表现出脑室扩大征象；至第4周表现为明显的脑室扩大和皮层变薄。实验组大鼠在Morris水迷宫实验中无法准确找到平台；训练第4、5、6天时，实验组大鼠逃避潜伏期明显长于两个对照组（P<0.01）。结论经枕大池注射白陶土可以有效地建立梗阻性脑积水动物模型。

关键词: 脑积水, 白陶土, Morris水迷宫, 新生大鼠

Abstract:

Objective To establish newborn rat model of obstructive hydrocephalus by kaolin suspension injection. Methods The newborn rats (aged 1 d) were injected with kaolin suspension through cistern magna (experiment group, n=40), and saline control group (n=10) and normal control group (n=10) were also established. Rats in experiment group were divided into four subgroups (n=10), and were observed 1 week, 2 weeks, 3 weeks and 4 weeks after injection, respectively. Rats underwent MRI examinations 1 week, 2 weeks, 3 weeks and 4 weeks after injection. Space learning and memory ability was assessed with Morris water maze tests when rats aged one month. Results Rats in experiment group exhibited ataxic behaviors such as hunch back and wide based gait. One week after kaolin injection, cerebral ventricle enlargement was observed by MRI in rats of experiment group, and significant cerebral ventricle enlargement and atrophy of cortex parenchyma were revealed at the 4th week. Rats in experiment group could not find platform accurately in Morris water maze tests, and the escape latency of rats in experiment group was significantly longer than that in saline control group and normal control group at the 4th day, 5th day and 6th day of training (P<0.01). Conclusion Kaolin injection through cistern magna can effectively establish rat model of obstructive hydrocephalus.

Key words: hydrocephalus, kaolin, Morris water maze, newborn rat

吴少峰, 杨波, 金惠明, 等. 白陶土诱导新生大鼠梗阻性脑积水模型的建立[J]. , 2011, 31(1): 35-.

WU Shao-feng, YANG Bo, JIN Hui-ming, et al. Establishment of newborn rat model of obstructive hydrocephalus by kaolin injection[J]. , 2011, 31(1): 35-.

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白陶土诱导新生大鼠梗阻性脑积水模型的建立

Establishment of newborn rat model of obstructive hydrocephalus by kaolin injection

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