上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (8): 1105-.doi: 10.3969/j.issn.1674-8115.2011.08.013

• 专题报道（眼科视觉科学） • 上一篇    下一篇

三种方法对大鼠视网膜固定效果的比较研究

李晶晶, 朱 鸿, 施彩虹   

  1. 上海交通大学 医学院附属第三人民医院眼科, 上海 201900
  • 出版日期:2011-08-28 发布日期:2011-08-29
  • 通讯作者: 施彩虹, 电子信箱: schhys@yahoo.com.cn。
  • 作者简介:李晶晶（1985—）, 女, 硕士生；电子信箱: ljjandsm@gmail.com。
  • 基金资助:

    上海市科学技术委员会（10ZR1418500）

Outcomes of rat retina fixation with three different methods

LI Jing-jing, ZHU Hong, SHI Cai-hong   

  1. Department of Ophthalmology, the Third People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201900, China
  • Online:2011-08-28 Published:2011-08-29
  • Supported by:

    Shanghai Science and Technology Committee Foundation, 10ZR1418500

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摘要：

目的 研究三种不同方法对大鼠视网膜石蜡切片的固定效果。方法 根据离体眼球的不同固定方法，将9只SD大鼠随机分为A组（改良固定液固定，n=3）、B组（4%多聚甲醛固定，n=3）和C组（4%多聚甲醛心脏灌注法固定，n=3）。24 h后制作标本进行苏木精-伊红染色，分析比较固定效果。结果 A组固定的眼杯不变形，B组固定的有少许变形，C组固定的基本不变形。显微镜观察显示A组眼球视网膜各层组织结构完整，B组和C组视网膜均有断层、分离和脱片等现象。结论 改良固定液固定眼球的方法优于单纯4%多聚甲醛和4%多聚甲醛心脏灌注固定的方法，适用于视网膜石蜡切片的后期研究。

关键词: 眼球固定液, 石蜡切片, 大鼠视网膜

Abstract:

Objective To investigate the outcomes of fixation of rat retina paraffin sections with three different methods. Methods Nine SD rats were randomly divided into group A (retina fixation with improved solution, n=3), group B (retina fixation with 4% paraformaldehyde, n=3) and group C (retina fixation with cardiac perfusion of 4% paraformaldehyde, n=3). Paraffin sections were prepared 24 h after retina fixation, HE staining was performed, and the outcomes of fixation were compared. Results There was no eyecup deformation in group A,  a few eyecup deformations in group B and few eyecup deformation in group C. It was observed by microscopy that each layer of the retina structure was intact in group A,  and there was retinal detachment in group B and group C. Conclusion Eyeball fixation with improved fixation solution may result in better outcomes than fixation with 4% paraformaldehyde or fixation with cardiac perfusion, and is suitable for further research with retinal paraffin sections.

Key words: eyeball fixation solution, paraffin section, rat retina