上海交通大学学报（医学版）

›› 2011, Vol. 31 ›› Issue (12): 1692-.doi: 10.3969/j.issn.1674-8115.2011.12.007

• 论著（基础研究） • 上一篇    下一篇

白介素12在γδT细胞表达L-选择素中的调控作用

武文娟1, 耿英华2, 石玉荣1, 李柏青3   

  1. 1.蚌埠医学院生物化学与分子生物学教研室, 蚌埠 233030； 2.蚌埠医学院第一附属医院血液科, 蚌埠 233030； 3.蚌埠医学院免疫学教研室, 蚌埠 233030
  • 出版日期:2011-12-28 发布日期:2012-01-04
  • 作者简介:武文娟（1973—）, 女, 副教授, 硕士, 硕士生导师；电子信箱: wuwj_2011@126.com。
  • 基金资助:

    安徽省高等学校省级自然科学研究项目(KJ2011B098) ；安徽省高等学校青年教师科研资助计划（2007jq1132）

Role of interleukin-12 in regulation of expression of L-selectin on γδT cells

WU Wen-juan1, GENG Ying-hua2, SHI Yu-rong1, LI Bai-qing3   

  1. 1.Department of Biochemistry and Molecular Biology, Bengbu Medical College, Bengbu 233030, China；2.Department of Hematology, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233030, China|3.Department of Immunology, Bengbu Medical College, Bengbu 233030, China
  • Online:2011-12-28 Published:2012-01-04
  • Supported by:

    Provincial Natural Science Research Project of Anhui Colleges, KJ2011B098；Research Foundation for Young Teachers in Colleges of Anhui Province, 2007jq1132

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摘要：

目的 观察白介素12（IL-12）对γδT细胞表面L-选择素（CD62L）表达的调控作用。方法 人外周血活化γδT细胞经诱导及扩增后，采用Mtb-Ag首次刺激和再次刺激，在细胞分孔培养时分别加入不同的细胞因子（IFN-γ、IL-12+抗IFN-γ、IL-12+抗IL-12、IL-12+ IFN-γ）和不同浓度（0、0.1 ng/mL、1 ng/mL）IL-12。收集各组培养第0、3、5、7天的细胞，流式细胞术检测γδT细胞表面CD62L的表达。结果 Mtb-Ag首次刺激和再次刺激后，IL-12组、IL-12+抗IFN-γ组和IL-12+IFN-γ组γδT细胞表面CD62L的表达量均较空白对照组显著升高，差异有统计学意义（P<0.05）；而IFN-γ组、IL-12+抗IL-12组的CD62L表达量与空白对照组比较，差异无统计学意义（P>0.05）。随着IL-12浓度的增加，γδT细胞表面CD62L的表达量增加，其中Mtb-Ag首次刺激后第3和5天时，0.1 ng/mL和1.0 ng/mL IL-12组CD62L表达量均明显高于阴性对照组（P<0.05），且1.0 ng/mL IL-12组明显高于0.1 ng/mL IL-12组（P<0.05），具有浓度依赖性；Mtb-Ag再刺激后CD62L的表达量下调，但在加入IL-12后CD62L表达的下调速度减缓，且1.0 ng/mL IL-12比0.1 ng/mL IL-12更能使CD62L表达维持在相对较高水平（P<0.05）。结论 IL-12在γδT细胞活化时调控CD62L的表达并且具有浓度依赖性。

关键词: 白介素12, γδT细胞, L-选择素

Abstract:

Objective To investigate the role of interleukin (IL)-12 in regulation of the expression of L-selectin (CD62L) on γδT cells. Methods After induction and proliferation, human peripheral activated γδT cells were primarily and secondarily stimulated with Mtb-Ag. Cells were cultured with different cytokines (IFN-γ, IL-12+anti-IFN-γ, IL-12+anti-IL-12 and IL-12+IFN-γ) and different concentrations of IL-12 (0 ng/mL, 0.1 ng/mL and 1 ng/mL), respectively. Cell cultured for 0 d, 3 d, 5 d and 7 d were collected, and the expression of CD62L on γδT cells was detected by flow cytometry. Results After primary and secondary stimulation with Mtb-Ag, the expression of CD62L on γδT cells in IL-12 group, IL-12+anti-IFN-γ group and IL-12+ IFN-g group was significantly higher than that in blank control group (P<0.05), while the expression of CD62L on γδT cells in IFN-γ group and IL-12+anti-IL-12 group was not significantly different from that in blank control group (P>0.05). With the increase of concentrations of IL-12, the expression of CD62L on γδT cells increases. On the third day and fifth day after primary stimulation with Mtb-Ag, the expression of CD62L in 0.1 ng/mL IL-12 group and 1.0 ng/mL IL-12 group was significantly higher than that in negative control group (P<0.05), and the expression of CD62L in 1.0 ng/mL IL-12 group was significantly higher than that in 0.1 ng/mL IL-12 group (P<0.05). The expression of CD62L decreased after secondary stimulation with Mtb-Ag, while the decrease of expression of CD62L slowed down after treatment with IL-12, and the effect was more significant for 1.0 ng/mL IL-12 compared with 0.1 ng/mL IL-12 (P<0.05). Conclusion The expression of CD62L is dose-dependently regulated by IL-12 when γδT cells are activated.

Key words: interleukin-12, γδT lymphocytes, L-selectin