目的·筛选耐药肺炎克雷伯菌的噬菌体，并检验其是否符合耐药肺炎克雷伯菌噬菌体库的入库要求。方法·将分离自1例泌尿系统感染患者的1株耐药肺炎克雷伯菌作为宿主菌，从环境污水中进行噬菌体筛选，分离、纯化噬菌体并进行生物学特性的研究及噬菌体基因组测序，并基于全基因组数据分析其使用安全性。结果·分离到1株耐药肺炎克雷伯菌噬菌体，将其命名为JD902，属有尾目，裂解量为88 pfu/cell，在pH值4.0～11.0范围内都能保持较好的活性。基因组分析表明，其不携带抗生素抗性基因和毒力因子基因。结论·JD902为1株烈性噬菌体，体外稳定性及安全性均较高，可以纳入临床治疗使用噬菌体库。

Objective · To screen for a bacteriophage of a drug-resistant Klebsiella pneumoniae strain and assess its eligibility to be incorporated into the Klebsiella bacteriophage library. Methods · A drug-resistant Klebsiella pneumoniae strain isolated a patient with urinary infection was used as the host strain. Phage screening was carried out in environmental sewage, and the bacteriophage was isolated, purified, characterized and sequenced. Safety evaluation was also conducted based on the genome sequence. Results · A drug-resistant Klebsiella pneumoniae bacteriophage was isolated and designated as JD902, which belonged to Caudovirales. The burst size was 88 pfu/cell, and it could maintain good activity in pH range 4.0-11.0. Genomic analysis demonstrated that it did not carry any antibiotic resistance genes and virulence factor genes. Conclusion · JD902 is a virulent phage with high stability and safety in vitro. It can be incorporated into bacteriophage library for clinical treatment.