›› 2019, Vol. 39 ›› Issue (9): 933-.doi: 10.3969/j.issn.1674-8115.2019.09.001

• Original article (Basic research) • Previous Articles     Next Articles

Liver metabonomics analysis of olanzapine-induced weight gaining in rats

WANG Ye-wei, FANG Xin-yu, CHEN Yan, WANG Dan-dan, YU Ling-fang, ZHANG Chen   

  1. Biochemical Laboratory, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China
  • Online:2019-09-28 Published:2019-11-02
  • Supported by:
    National Natural Science Foundation of China, 81000581, 81471358, 81771450; National Key Research and Development Program, 2018YFC1314302; National Key Clinical Discipline at Shanghai Mental Health Center, OMA-MH 2011-873; Shanghai Science and Technology Development Fund, 12140904200; Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Support, 20152530)。

Abstract: Objective · To observe the changes in metabolites and metabolic pathways in SD rats after olanzapine treatment. Methods · Twenty female SD rats were randomly assigned to two groups, control group and olanzapine treatment group, respectively. During the treatment period, body weight and food intake of two groups were monitored. At the end of treatment, the liver tissues were collected and LC/MS technique was applied to detect metabolites in liver tissue. Subsequently, the raw data were converted for further analysis on the MetaboAnalysis platform. Results · Short-term olanzapine administration resulted in significant increases in body mass, food intake, and impaired glucose tolerance. Metabonomics results indicated rats olanzapine treatment group had a significantly higher level of primary bile acid and 9-OxoODE (9-oxo-octadecadienoic acid) in liver tissue than those control group. Conclusion · Primary bile acid biosynthesis process is significantly enhanced after short-time olanzapine treatment.

Key words: olanzapine, metabonomics, liver tissue, liquid chromatography-mass spectrometry

CLC Number: