›› 2009, Vol. 29 ›› Issue (12): 1424-.

• Original article (Basic research) • Previous Articles     Next Articles

Expression change of stromal cell-derived factor 1alpha in retinas after partial optic nerve injury

PAN Dong-chao1, BI Yong-yan1, FENG Dong-fu2, CHEN Er-tao1, CHU Sheng-hua2, WANG Yang3   

  1. 1. Department of Neurosurgery, 2. Department of Neurosurgery, Institute of Traumatic Medicine, The Third People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 201900, China；3. Shanghai Medicine School, Fudan University, Shanghai 200032, China
  • Online:2009-12-25 Published:2009-12-25
  • Supported by:

    Shanghai Education Committee Foundation, 04BB23,07ZZ42; Natural Science Foundation of Shanghai, 09ZR1417500

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Abstract:

Objective To investigate the changes of expression of stromal cell-derived factor 1α (SDF-1α) in retinas after partial optic nerve injury in rats. Methods Models with injury of partial optic nerve were induced in rats. Retinal tissues were collected 1,2,3,5,7,10 and 14 d after injury. Enzyme linked immunosorbent assay and Real-time quantitative PCR were employed to detect the expression of SDF-1α protein and mRNA in retinal tissues respectively in injury group （n=28）, sham operated group （n=28） and normal control group （n=12）. Results The expression of SDF-1α protein and mRNA in retinas was higher than that in sham operated group and normal control group at different time points after injury (P<0.01), and it reached the peak at the 5th day after injury. The expression of SDF-1α protein and mRNA maintained a high level at the 14th day after injury. Conclusion The expression of SDF-1α protein and mRNA is up-regulated after partial optic nerve injury, and may last for a long time.

Key words: optic nerve injury, retina, stromal cell-derived factor 1α