›› 2010, Vol. 30 ›› Issue (6): 703-.

• Original article (Clinical research) • Previous Articles     Next Articles

Expression of single-stranded DNA in peripheral blood mononuclear cells in patients with systemic lupus erythematosus

ZHAO Bao-jing, HUANG Xin-fang, SHEN Nan, CHEN Shun-le, WANG Yuan   

  1. Department of Rheumatology,Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200001, China
  • Online:2010-06-25 Published:2010-06-28
  • Supported by:

    National Natural Science Foundation of China, 30301026；Shanghai Key Discipline Construction Project, T0203；Shanghai Excellent Academic Leader Plan, 07XD14021；Shanghai Clinical Research Resource Sharing Platform Project, SHDC12007205

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Abstract:

Objective To investigate the expression of single-stranded DNA (ssDNA) in peripheral blood mononuclear cells (PBMC) in patients with systemic lupus erythematosus (SLE). Methods Flow cytometry was employed to detect the percentages of PBMC with positive ssDNA expression in 26 patients with SLE (SLE group), and normal control group (n=17) and the other connective tissue diseases (CTD) control group (n=7) were established. Results The ssDNA positive rates in peripheral B lymph cells in SLE group, normal control group and CTD control group were (0.906±0.838)%, (0.216±0.371)% and (0.270±0.311)%, respectively. The ssDNA positive rate in peripheral B lymph cells in SLE group was significantly higher than those in normal control group and CTD control group (P<0.01, P<0.05). There were significant differences in the ssDNA positive rates in peripheral B lymph cells between patients with lupus nephritis in SLE group and those without lupus nephritis in SLE group [(1.228±0.259)% vs (0.510±0.436)%, P<0.05]. Conclusion The development of SLE and lupus nephritis may be related to the increase of peripheral B lymph cells with ssDNA expression.

Key words: single-stranded DNA, peripheral blood mononuclear cell, systemic lupus erythematosus, lupus nephritis