›› 2011, Vol. 31 ›› Issue (5): 684-.doi: 10.3969/j.issn.1674-8115.2011.05.034

• Technique and method • Previous Articles     Next Articles

Effect of different 16S rDNA universal primers on DGGE patterns of periodontal microbial community

ZHOU Yan-bin, SHU Rong, LIU Da-li   

  1. Department of Periodontology, the Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China
  • Online:2011-05-28 Published:2011-05-27
  • Supported by:

    National Science and Technology Foundation of the Eleventh Tenth-Five Year Plan, 2007BAI18B02；Shanghai Key Discipline Construction Program, S30206；Shanghai Education Committee Foundation, 09YZ78

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Abstract:

Objective To preliminarily assess the effect of different 16S rDNA fragments on denaturing gradient gel electrophoresis (DGGE) patterns of periodontal microbial community. Methods Three typical periodontal bacterial strains were selected, plasmid DNA was constructed to get 16S rDNA V3, V3-V5 and V6-V8 region fragments, and sequencing analysis and BLAST identification were performed. The bands of strains were obtained after DGGE isolation and silver nitrate staining of sequenced plasmid DNA. Results DGGE profiles by three universal primers were different. The bands of 16S rDNA V3 region fragments of typical strains were not single, and bands of different strains could not differ from each other. The single band of 16S rDNA V3-V5 and V6-V8 region fragments of each typical strain was found. Conclusion 16S rDNA V3-V5 and V6-V8 region fragments are suitable for periodontal bacteria structure analysis.

Key words: subgingival bacteria community, 16s rDNA, denaturing gradient gel electrophoresis, putative pathogen