Abstract:

Objective To observe the effects of heart preservation solution containing different concentrations of Pinacidil on donor heart preservation. Methods Forty SD rats were randomly divided into normal control group, Celsior solution control group, Celsior solution+0.25 mmol/L Pinacidil group, Celsior solution+0.5 mmol/L Pinacidil group and Celsior solution+1 mmol/L Pinacidil group according to different ways of donor heart preservation, with 8 rats in each group. Langendorff model of isolated heart was established, and isolated hearts were preserved in preservation solutions for 6 h, followed by reperfusion for 30 min. The parameters of cardiac function such as left ventricular end-systolic pressure (LVESP), left ventricular development pressure (LVDP), coronary flow (CF), maximum rate of left ventricular pressure rise (dp/dtmax) and minimum rate of left ventricular pressure rise (dp/dtmin), myocardial ultrastructure and myocardiocyte apoptotic index were observed and compared among groups. Results Compared with Celsior solution control group, Celsior solution+0.25 mmol/L Pinacidil group and Celsior solution+1 mmol/L Pinacidil group, the parameters of LVESP, LVDP, CF, dp/dtmax and dp/dtmin significantly increased (P＜0.05), the myocardiocyte apoptotic index significantly decreased (P＜0.05), and the structures of myocardiocytes and mitochondria were intact in Celsior solution+0.5 mmol/L Pinacidil group. Conclusion Compared with single Celsior heart preservation solution, Celsior heart preservation solution containing 0.5 mmol/L Pinacidil can decrease myocardiocyte apoptosis after donor heart preservation, promote recovery of cardiac function and improve the effects of hypothermic preservation of donor hearts.

Key words: Pinacidil, heart preservation, cardiomyocyte apoptosis, ischemia-reperfusion injury