›› 2018, Vol. 38 ›› Issue (1): 18-.doi: 10.3969/j.issn.1674-8115.2018.01.004

• Original article (Basic research) • Previous Articles     Next Articles

Function of OTUD7B in acute myeloid leukemia cells and its mechanism

GONG Xiu-feng, WU Ying-li, ZHAO Qian, LEI Hu   

  1. Department of Pathophysiology, The Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
  • Online:2018-01-28 Published:2018-03-09
  • Supported by:
    National Natural Science Foundation of China, 81700475,
    81570118; Project of Science and Technology Committee of Shanghai Municipality, 15401901800

Abstract: Objective · To investigate the biological effect and mechanism of OTUD7B in acute myeloid leukemia (AML) cells. Methods · The expression
of OTUD7B in peripheral blood mononuclear cells and bone marrow mononuclear cells of AML patients were detected. The relationship between OTUD7B
and survival of AML patients was confirmed by using TCGA database. Mouse model of M2 type AML was established, and the expression of OTUD7B in
the bone marrow, spleen and liver of the mice was detected. OTUD7B was overexpressed in AML cell lines HL60 and kasumi1, then the cell viability and
cell cycle were measured. The AKT/mTOR pathway proteins were detected after OTUD7B overexpressed and then the cell growth inhibition was detected
after overexpression of AKT1. Results · The expression of OTUD7B was lower in primary leukemia cells from all types of AML patients and in the bone
marrow, liver and spleen of M2 type AML mice, which was closely related to the survival time of AML patients. OTUD7B overexpression in HL60 and
kasumi1 cells significantly inhibited the cell viability and decreased the percentage of S phase cells. OTUD7B significantly inhibited the phosphorylation of
AKT and mTOR, and AKT1 overexpression partially reversed the inhibitory effect of OTUD7B on cell growth. Conclusion · OTUD7B expression is low in
primary leukemia cells from AML patients and in bone marrow, liver and spleen of the M2 type AML mice. The survival time of patients with low OTUD7B
expression is shorter. Overexpression of OTUD7B significantly inhibited the cell viability of HL60 and kasumi1 cells and the entry of cells into S phase. The
inhibitory effect of OTUD7B overexpression on AML cells might be related to the inhibition of AKT / mTOR signaling pathway.

Key words: OTUD7B, acute myeloid leukemia, proliferation inhibition, AKT/mTOR signaling pathway