ATP-sensitive potassium channel negatively regulates hippocampal long-term potentiation maintenance

doi:10.3969/j.issn.1674-8115.2021.02.005

JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE) ›› 2021, Vol. 41 ›› Issue (2): 154-158.doi: 10.3969/j.issn.1674-8115.2021.02.005

• Basic research • Previous Articles Next Articles

ATP-sensitive potassium channel negatively regulates hippocampal long-term potentiation maintenance

Xiao-lin ZHANG(), Xiao-yun ZHANG, Gui-qin HE, Yue KONG, Zi-kai ZHOU()

Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China

Received:2020-03-30 Online:2021-02-28 Published:2021-02-28
Contact: Zi-kai ZHOU E-mail:1056372478@qq.com;zikai.zhou@live.com
Supported by:
Shanghai Municipal Education Commission—Gaofeng Clinical Medicine Grant Support(20191835);Program of Shanghai Academic Research Leader(19XD1423300)

Abstract

Abstract: Objective

·To investigate the regulatory roles of ATP-sensitive potassium channel (K_ATP) in the induction and maintenance stages of long-term potentiation (LTP), which is an experimental model for studies of synaptic plasticity and memory.

Methods

·Acute brain slices were prepared from male C57BL/6 mice at 5?6 weeks of age. Electrophysiological recording of the field excitatory postsynaptic potentials was performed at the hippocampal Schaffer collateral-commissural pathway. K_ATP opener cromakalim was used to activate K_ATP at basal level, induction and maintenance stages of LTP. Oxygen-glucose deprivation (OGD) was used to induce hypoxia and pathological opening of K_ATP at the maintenance stage of LTP. Tolbutamide (TOL) was used to block K_ATP before OGD treatment.

Results

·K_ATP opening showed no effects on basal synaptic transmission and the induction of LTP, but largely decreased the magnitude of LTP at maintenance stage. OGD rapidly impaired LTP maintenance, which was significantly prevented by TOL pre-treatment.

Conclusion

·K_ATP negatively regulates hippocampal LTP maintenance in both physiological and pathological conditions.

Key words: ATP-sensitive potassium channel (K_ATP), long-term potentiation (LTP), synaptic plasticity

CLC Number:

R338

Xiao-lin ZHANG, Xiao-yun ZHANG, Gui-qin HE, Yue KONG, Zi-kai ZHOU. ATP-sensitive potassium channel negatively regulates hippocampal long-term potentiation maintenance[J]. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), 2021, 41(2): 154-158.

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References 16

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ATP-sensitive potassium channel negatively regulates hippocampal long-term potentiation maintenance

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