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Influence of HPV16 E2 on epithelial-mesenchymal transition of cervical cancer SiHa cells

QIAN Wen-yan1,2, ZHANG Jia-wen1, DONG Yu1, LIU Yuan1, SHI Can1, ZHANG Zhen-bo1, YANG Yong-bin1, FENG You-ji1, WU Su-fang1   

  1. 1.Department of Obstetrics and Gynecology, the First People's Hospital, Shanghai Jiaotong University, Shanghai 201620, China; 2.Kunshan Hospital of Traditional Chinese Medicine, Kunshan 215300, China
  • Online:2013-08-28 Published:2013-09-16
  • Supported by:

    Shanghai Science and Technology Committee Foundation, 09411962500, 12PJD002; National Natural Science Foundation of China, 81201541; Foundation of the Ministry of Health of China, 201002013

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Abstract:

Objective To investigate the influence of HPV16 E2 on epithelial-mesenchymal transition of cervical cancer SiHa cells. Methods Lipofectamine was used to transfect p-CAG-MYC-HPV16 E2 gene into cervical cancer SiHa cells. The morphology of cells was observed before and after transfection with phase-contrast microscope, and the expression of Twist2, E-cadherin and Vimentin before and after transfection was determined by RT-PCR and Western blotting. Results HPV16 E2 was successfully transfected into SiHa cells. After transfection, the epithelial cells became spindle-shaped, with enlarged intercellular space, and the expression of Twist2 and Vimentin was up-regulated, while the expression of E-cadherin was down-regulated. Conclusion HPV16 E2 can induce epithelialmesenchymal transition of cervical cancer cells, and promote cervical carcinogenesis.

Key words: HPV16 E2, epithelial-mesenchymal transition, transfection, cervical cancer