• Original article (Clinical research) • Previous Articles     Next Articles

Effect of anti-tuberculosis drug-induced hepatotoxicity on plasma microRNA expression

YE Chang-gen, XIE Ping, LIU Liang-ming, BAI Ru, CHEN Cai-ping, ZHU Wei-xing, ZHAO Hui, WANG Ying-ying, WANG Yan-yan, WU Jian-qin, GAO De-yong, XU Guo-rong, XIA Li-ping   

  1. Department of Infectious Diseases, Songjiang Hospital Affiliated to the First People′s Hospital, Shanghai Jiao Tong University, Shanghai 201600, China
  • Online:2014-02-28 Published:2014-03-25
  • Supported by:

    National Natural Science Foundation of China, 81070357, 30660066; Leading Medical Cooperation Project of Songjiang District of Shanghai, 2011LX02

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Abstract:

Objective To investigate the effect of anti-tuberculosis drug-induced hepatotoxicity (ATDH) on the expression of miRNA in patients′ plasma. Methods The plasma from 3 patients with ATDH was collected and subjected to miRNA microarray analysis before anti-tuberculosis treatment and after liver injury during drug therapy. The differentially expressed miRNAs were verified using real-time quantitative PCR. The target genes of miRNAs were predicted by the Internet software, and the GO functional classification of target proteins were found by the PANTHER protein classification system. Results After ATDH had occurred, the microarray screened 22 differentially expressed miRNAs compared to those of pretreatment, among which 11 were up-regulated, and 11 were down-regulated. Real-time quantitative PCR results showed that after ATDH there were 5 up-regulated miRNAs, namely miR-378i, miR-125b-5p, miR-1224-5p, miR-194-5p and miR-34a-5p, and 3 down-regulated miRNAs, namely miR-1260a, miR-338-3p and miR-4286. Conclusion There are differentially expressed miRNAs in the plasma of patients after ATDH, and these miRNAs may be related to the incidence of ATDH.

Key words: anti-tuberculosis drug, hepatotoxicity, microRNA, plasma, differentially expressed