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Effects of andrographolide on proliferation and apoptosis of NB4 cells in vitro

TANG Yu-hong, WANG Yu-li, LU Jing-feng, ZHANG Yan-fang, SHEN Guo-yong   

  1. Department of Hematology, Shanghai Third People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201999, China
  • Online:2015-01-28 Published:2015-01-29
  • Supported by:

    Scientific Research Foundation of Shanghai Outstanding Young University Teachers Cultivation, ZZjdyx12115； Natural Science Foundation of Shanghai Third People's Hospital, Shanghai Jiao Tong University School of Medicine, syz2011-11

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Abstract:

Objective To investigate the effects of andrographolide (AD) on the cell proliferation and apoptosis of human acute promyelocytic leukemia-NB4 cells in vitro. Methods NB4 cells were treated with AD of different concentrations for 48 h. The cell proliferation was detected by the MTT assay and the early apoptosis rate was determined by the AnnexinV/PI double staining. The subdiploid peak and cell cycle were analyzed by the DNA-PI staining. The expressions of apoptosis related proteins were detected by the Western blotting. Results AD of 5-25 μmol/L significantly inhibited the proliferation of NB4 cells. The half inhibit concentration (IC50) of AD at 48 h was (1.98±0.22) μmol/L. With the increasing of AD concentration, the early apoptotic rate, subdiploid peak proportion, and apoptosis proportion elevated significantly (P<0.05). The results of Western blotting showed that the expressions of apoptosis related proteins were significantly activated. Conclusion AD can effectively inhibit the proliferation of NB4 cells, induce cell apoptosis, and arrest the cell cycle.

Key words: andrographolide, NB4 cells, proliferation inhibition, apoptosis