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Analysis of the correlation between the polymorphism of locus rs498422 in HLA-DRA gene of Chinese Han population and the hereditary susceptibility #br# of non-obstructive azoospermia

SONG Ping-ping1, ZOU Sha-sha1, YANG Juan-juan2, SHI Yong-yong2, DU Yan-zhi1, HU Hong-liang1   

  1. 1.Center for Reproduction Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Human Sperm Bank, Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai 200135,China; 2.BIO-X Center, Shanghai Jiao Tong University, Shanghai 200030, China
  • Online:2016-01-28 Published:2016-02-26
  • Supported by:

    Foundation of Renji Hospital， Shanghai Jiao Tong University School of Medicine， RJZZ14-017； National Natural Science Foundations of China， 30973069， 30672146。

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Abstract:

Objective To explore the correlation between the polymorphism of locus rs7194 in HLA-DRA gene of Chinese Han population and the hereditary susceptibility of non-obstructive azoospermia (NOA).  Methods The method of direct sequencing of PCR products was used for genotyping of 275 patients with NOA (case group) and 221 healthy controls (control group). The genotypes and distribution frequencies of alleles of two groups were compared. Logistic regression analysis was adopted to explore the susceptible genotypes of NOA. The meta-analysis was used to further verify the correlation between the polymorphism of locus rs7194 and NOA.  Results The differences of frequencies of genotypes and alleles of locus rs7194 between two groups were statistically significant (P=0.003 and P=0.001, respectively). Results of the logistic regression analysis showed that genotypes A/G and G/G correlated with the elevated incidence of NOA (OR=1.837, P=0.002; OR=1.942, P=0.048). Results of the meta analysis also indicated that the polymorphism of locus rs7194 in HLA-DRA gene correlated with NOA. Conclusion The polymorphism of locus rs7194 in HLA-DRA gene correlates with the hereditary susceptibility of NOA of Chinese Han population. The incidence of NOA of people carrying G allele with genotype A/G or G/G elevates. Genotypes A/G and G/G may be risk factors of the incidence of NOA.

Key words: non-obstructive azoospermia, single nucleotide polymorphism, HLA-DRA gene