Abstract:

Objective To investigate the effects of triclosan on expression of 11β-HSD2 and HLA-G in human first trimester trophoblasts. Methods First trimester trophoblasts were isolated and purified with modified Kliman method, including villous cytotrophoblasts and extravillous cytotrophoblasts. Villous cytotrophoblasts fused to form syncytiotrophoblasts during culture in vitro. First trimester trophoblasts were treated with different concentrations of triclosan (0, 0.000 1, 0.001, 0.01, 0.1, and 1 μmol/L) for 24 h after three days of culture. The expression of 11β-HSD2 in syncytiotrophoblasts and HLA-G in extravillous cytotrophoblasts was detected by Western blotting. The gel zymography was used to detect the enzymatic activity of MMP2/9 in extravillous cytotrophoblasts. Results The expression of 11β-HSD2 in syncytiotrophoblasts and HLA-G in extravillous cytotrophoblasts was significantly decreased and the enzymatic activity of MMP2/9 in extravillous cytotrophoblasts was reduced after triclosan treatment. Conclusion Triclosan can inhibit the expression of 11β-HSD2 and HLA-G and the  enzymatic activity of MMP2/9 in human first trimester trophoblasts, suggesting excessive exposure to triclosan may have adverse effects on the reproductive health in females.

Key words: triclosan, extravillous cytotrophoblast； syncytiotrophoblast, 11β-HSD2, HLA-G, MMP2/9