›› 2017, Vol. 37 ›› Issue (6): 738-.doi: 10.3969/j.issn.1674-8115.2017.06.005

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Effect of gambogic acid on NK/T-cell lymphoma

LI Gao-yang, JIANG Ji-feng, LIU Chuan-xu, TAO Rong   

  1. Department of Hematology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai  200092, China
  • Online:2017-06-28 Published:2017-07-05
  • Supported by:

     Shanghai Municipal Education Commission— Gaofeng Medicine Grant Support, 20152219; Project of Shanghai Municipal Education Commission, 13ZZ084


 Objective · To identify the effect and potential mechanism of gambogic acid (GA) on natural killer/T-cell lymphoma (NK/TCL) cell lines.  Methods · SNK-1, SNK-6 and SNT-8 were incubated with various concentrations of GA for 24 h, and cell viability was detected with CCK-8 assay. Cell apoptosis was examined by Annexin V-FITC/PI staining assay. Levels of proteins regulating cell apoptosis and phosphorylation levels of proteins in key signaling pathways were detected by Western blotting.  Results · GA showed a potent effect on reduction of cell viability of NK/TCL cell lines in CCK-8 assay. GA increased the percentages of Annexin V positive cells and induced activation of caspase-3 and caspase-9, cleavage of PARP as well as the reduction of Bcl-xl. GA also inhibited the phosphorylation levels of STAT3 in SNK-1 and SNT-8, and ERK1/2 in SNK-1 and SNK-6 significantly.  Conclusion · GA induces cell apoptosis in NK/TCL cell lines SNK-1, SNK-6 and SNT-8. Anti-apoptosis protein Bcl-xl and signaling pathway JAKs/ STATs and MEK/MAPK might be involved in this process.

Key words:  NK/TCL, gambogic acid, apoptosis