›› 2018, Vol. 38 ›› Issue (10): 1145-.doi: 10.3969/j.issn.1674-8115.2018.10.002

• Original article (Basic research) • Previous Articles     Next Articles

Metabolomics study of the effect of arsenic trioxide on hepatocellular carcinoma cell line HepG2

HUANG Jin-guo1*, ZHANG Hai-nan1*, CHEN Zi-qing1*, YANG Li-na2, GUO Shu-juan1, TAO Sheng-ce1   

  1. 1. Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China; 2. Department of Integrative Oncology, Fudan University Shanghai Cancer Center, Shanghai 200032, China
  • Online:2018-10-28 Published:2018-11-18
  • Supported by:
    National Key Research and Development Program of China, 2016YFA0500600; National Natural Science Foundation of China, 31670831

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Abstract: Objective · To reveal the mechanism of the effect of arsenic trioxide (ATO) on hepatocellular carcinoma (HCC) cells through metabolomics study. Methods · HCC cell line HepG2 was treated with ATO for 12 h, 24 h and 36 h respectively. The global metabolite profiles were monitoredmetabolomics analysis using GC/MS and LC/MS/MS. Results · A total of 307 certified metabolites were detected. Bioinformatics analysis showed that glycosylation and one-carbon metabolism were the main cellular functions that have been affected. Other affected cellular functions/pathways included mitochondrial function fatty acid β-oxidation, TCA cycle, pentose phosphate pathway (PPP) and glutathione metabolism. Conclusion · ATO treatment affects the main metabolomic pathway of HepG2, and inhibits cell growth and migration, then exacerbates oxidative stress to induce cancer cell apoptosis.

Key words: arsenic, arsenic trioxide, hepatocellular carcinoma, HepG2, metabolomics

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