Abstract: Objective · To investigate the changes of extracellular adenosine triphosphate (ATP) metabolism and its related indices of inflammatory response stage during diabetic wound healing. Methods · Ninety-six C57BL/6J mice were divided into 4 groups randomly, i.e. normal control group (NC, n24), normal with ATP applied group (NA, n24), diabetes control groups (DC, n24), and diabetes with ATP applied group (DA, n24). The mice in DA and DC group were inducedstreptozocin multiple intraperitoneal injection. Full-thickness excisional wound was created on the dorsum of all mice. Distilled water was applied in NC and DC group. ATP was applied in NA and DA group. Hematoxylin and eosin staining (HE staining) was used to observe neutrophils infiltration. Myeloperoxidase deficiency (MPO) contents were detectedenzyme-linked immunosorbent assay (ELISA). The contents of ATP and its metabolites in tissues of wound margin were detectedultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Immunohistochemistry was utilized to investigate the of Connexin43, Pannexin1, CD39, P2X7 and P2Y2. Healing speed were assessedthe calculation of healing rate on each point. Results · ①During the inflammatory response stage of wound healing, the MPO contents, neutrophils infiltration numbers, the ATP contents, and the s of Connexin43, Pannexin1, CD39, P2X7 and P2Y2 of mice in DC group were all lower than those in NC group (all PP0.000). Conclusion · Inflammatory response is insufficient on the early stage of diabetic wound healing, while ATP, ATP hydrolase and purinergic receptor also remain at low level at the same time. To a certain extent, exogenous application of ATP can accelerate diabetic wound healing rate.

Key words: diabetes mellitus, adenosine triphosphate (ATP), inflammation, wound healing