Journal of Shanghai Jiao Tong University (Medical Science)
MA Xiuzhen1,2(), ZHOU Ni3(
), GUO Siqi2,4, WANG Yuanyuan2,5, MAI Ping1,2(
)
Received:
2024-07-04
Accepted:
2024-10-28
Contact:
MAI Ping
E-mail:2060935938@qq.com;z18355425311@163.com;maiping_lz@163.com
Supported by:
CLC Number:
MA Xiuzhen, ZHOU Ni, GUO Siqi, WANG Yuanyuan, MAI Ping. Cannabinoid receptor 1 promotes M1 polarization of macrophages through the Gαi/o/RhoA signaling pathway in mice with acute lung injury[J]. Journal of Shanghai Jiao Tong University (Medical Science).
Gene | Forward primer (5'→3') | Reverse primer (5'→3') |
---|---|---|
18S rRNA | GTAACCCGTTGAACCCCATT | CCATCCAATCGGTAGTAGCG |
TNF-α | GGCAGGTTCTGTCCCTTTCA | CTGTGCTCATGGTGTCTTTTCTG |
IL-1β | CTACAGGCTCCGAGATGAACAAC | TCCATTGAGGTGGAGAGCTTTC |
IL-12 | CCTGAAGTGTGAAGCACCAAATT | CTTCAAGTCCATGTTTCTTTGCA |
Arg1 | AGCCACAGCTCTTCCCTTGA | CCTACCCCCGGAGAGTCACT |
Mrc2 | GAGCGACGGTCTAGGGTTTTC | GCCTCGGATATCATCGTCATC |
Mgl1 | AGAACAACGGCTCGGAAGTG | CCAATAGCAGCTGCCTTCATG |
Tab 1 Primer sequences for qPCR
Gene | Forward primer (5'→3') | Reverse primer (5'→3') |
---|---|---|
18S rRNA | GTAACCCGTTGAACCCCATT | CCATCCAATCGGTAGTAGCG |
TNF-α | GGCAGGTTCTGTCCCTTTCA | CTGTGCTCATGGTGTCTTTTCTG |
IL-1β | CTACAGGCTCCGAGATGAACAAC | TCCATTGAGGTGGAGAGCTTTC |
IL-12 | CCTGAAGTGTGAAGCACCAAATT | CTTCAAGTCCATGTTTCTTTGCA |
Arg1 | AGCCACAGCTCTTCCCTTGA | CCTACCCCCGGAGAGTCACT |
Mrc2 | GAGCGACGGTCTAGGGTTTTC | GCCTCGGATATCATCGTCATC |
Mgl1 | AGAACAACGGCTCGGAAGTG | CCAATAGCAGCTGCCTTCATG |
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