Abstract:

Objective To investigate the effects of perfluorochemical (PFC) on in vitro long-term hypothermic heart preservation in rats. Methods Thirty Wistar rats were randomly divided into control group (Celsior solution), Celsior+O₂ group (Celsior solution plus oxygen) and PFC/Celsior+O₂ group (two-layer method plus oxygen), with 10 rats in each group. Langendorff model of isolated rat heart was prepared. The isolated heart was preserved at 4℃ for 8 h, and hemodynamic parameters, coronary effluent flow, and leakage of creatine kinase (CK), lactate dehydrogenase (LDH) and aspartate transaminase (AST) were detected after reperfusion. Besides, the myocardial ultrastructure was also observed. Results Compared with control group and Celsior+O₂ group, the left ventricular developed pressure and ±dp/dt in PFC/Celsior+O₂ group significantly increased (P<0.01), while LDH, CK and AST leakage significantly decreased (P<0.01). However, there was no significant difference in the above paramters between Celsior+O₂ group and control group (P>0.05). Compared with control group and Celsior+O₂ group, the impairment of myocardial ultrastructure in PFC/Celsior+O₂ group after hypothermic preservation was alleviated. Conclusion PFC as a supplementation to oxygen in heart preservation solution could enhance myocardial protection during in vitro long-term hypothermic heart preservation in rats via the improvement of energy metabolism.

Key words: perfluorochemical, two-layer method, heart preservation, energy metabolism, rat