Abstract:

Objective To investigate the effects of different glucose concentrations and rosiglitazone intervention on expression of angiotension Ⅱ receptor 1 (AT1R) and angiotension Ⅱ receptor 2 (AT2R) genes in differentiated mature adipocytes. Methods Differentiated mature 3T3-L1 adipocytes were cultured in media with different concentrations of glucose (blank control, 5.6 mmol/L, 11.2 mmol/L and 25.0 mmol/L) and media with different concentrations of glucose and 10 nmol/L rosiglitazone for 24 h. Real-time PCR was employed to detect the expression of AT1R and AT2R genes in 3T3-L1 adipocytes. Results The expression of AT1R gene was significantly lower in 5.6 mmol/L glucose treatment group than that in blank control group, 11.2 mmol/L glucose treatment group and 25.0 mmol/L glucose treatment group (P<0.05), and the expression of AT2R gene significantly increased with the increase of glucose concentrations (P<0.05). The expression of AT1R and AT2R genes in rosiglitazone intervention group decreased with the increase of glucose concentrations. Compared with corresponding glucose treatment groups, the expression of AT1R gene in rosiglitazone intervention groups was significantly up-regulated (P<0.05), with the effect decreasing gradually with the increase of glucose concentrations, and the expression of AT2R gene was significantly down-regulated (P<0.05), with the effect increasing with the increase of glucose concentrations. Conclusion Rosiglitazone plays a regulatory role in the expression of AT1R and AT2R genes in adipocytes under high concentrations of glucose.

Key words: glucose, angiotesin Ⅱ receptor, rosiglitazone, adipocytes